DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

Hirofumi Sato; Yusuke Hashimoto; Mayuko Kikkawa; Takehiko Wada; Yoshihisa Inoue

DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

Hirofumi Sato, Yusuke Hashimoto, Mayuko Kikkawa, Takehiko Wada, Yoshihisa Inoue

IMRAM - Division of Organic- and Biomaterials Research

Osaka University

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

Original language	English
Pages (from-to)	159-160
Number of pages	2
Journal	Nucleic acids research. Supplement (2001)
Issue number	2
Publication status	Published - 2002

Cite this

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title = "DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.",

abstract = "Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.",

author = "Hirofumi Sato and Yusuke Hashimoto and Mayuko Kikkawa and Takehiko Wada and Yoshihisa Inoue",

year = "2002",

language = "English",

pages = "159--160",

journal = "Nucleic acids research. Supplement (2001)",

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T1 - DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

AU - Sato, Hirofumi

AU - Hashimoto, Yusuke

AU - Kikkawa, Mayuko

AU - Wada, Takehiko

AU - Inoue, Yoshihisa

PY - 2002

Y1 - 2002

N2 - Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

AB - Oligomeric peptide ribonucleic acids (PRNA's), tethering 5'-amino-5'-deoxyribonucleosides as a recognition site for nucleic acids, has been designed and synthesized by solid phase method. PRNA 12-mer found to form stable duplex with complementary DNA.PRNA.DNA duplex composed of PRNA with mismatched DNA has 10-degree lower melting temperature than fullmatched PRNA.DNA duplex. These results demonstrate that binding of PRNA with DNA is sequence specific. It should be emphasized that the on-off switching of PRNA.DNA duplex formation has been realized by the borate added as an external factor.

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M3 - Article

C2 - 12903154

AN - SCOPUS:0041818040

SP - 159

EP - 160

JO - Nucleic acids research. Supplement (2001)

JF - Nucleic acids research. Supplement (2001)

IS - 2

ER -

DNA recognition control of gamma-PRNA and mismatched base effects upon complex stability.

Abstract

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