Downregulated angiopoietin-like protein 8 production at calving related to changes in lipid metabolism in dairy cows

Misato Nakano, Yutaka Suzuki, Satoshi Haga, Eri Yamauchi, Dahye Kim, Koki Nishihara, Keiichi Nakajima, Takafumi Gotoh, Seungju Park, Myunggi Baik, Kazuo Katoh, Sanggun Roh

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


Acute physiological adaptation of lipid metabolism during the postpartum transition period of cows facilitates peripheral metabolic regulation. Hepatokines, which are hormones secreted from hepatocytes, are presumed to play a critical role in systemic metabolic regulation. Angiopoietin-like protein 8 (ANGPTL8) has been identified as a novel hepatokine associated with circulating triglyceride concentrations in mice and humans. However, regulation of ANGPTL8 and its physiological effects is still unknown in cattle. The present study aimed to reveal changes in ANGPTL8 expression and secretion during the periparturient period, and to investigate its regulatory effect on adipocytes and mammary epithelial cells. In the peripartum period, liver ANGPTL8 mRNA expression was lesser on the day of parturition and 1 wk postpartum than it was 1 wk before parturition (P < 0.05). Moreover, plasma ANGPTL8 concentrations decreased on the day of parturition as compared with that 1 wk before parturition (P < 0.05). In addition, ANGPTL8 expression in cultured bovine hepatocytes was downregulated after oleate and palmitate treatment but upregulated after insulin treatment (P < 0.05). ANGPTL8 decreased hormone-sensitive lipase (HSL) expression in differentiated adipocytes and cluster of differentiation 36 (CD36), fatty acid synthase (FAS), acetyl-coa carboxylase (ACC), and stearoyl-coa desaturase (SCD) in cultured bovine mammary epithelial cells (P < 0.05). These data suggest that hepatic ANGPTL8 production was downregulated postpartum when the cows experienced a negative energy balance. This downregula-tion was associated with increased concentrations of NEFA and decreased concentrations of insulin in lactating cows, and it facilitated lipid mobilization from adipose tissue to the mammary glands. We speculate that ANGPTL8 might have beneficial effects in reverting or improving the physiological adaptation and pathological processes of lipid metabolism during the peripartum period.

Original languageEnglish
Pages (from-to)2646-2658
Number of pages13
JournalJournal of animal science
Issue number7
Publication statusPublished - 2018 Jul 1
Externally publishedYes


  • Angiopoietin-like protein 8
  • Dairy cows
  • Hepatokine
  • Lipid metabolism
  • Negative energy balance

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics


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