Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea

Hiroshi Shiba; Jong In Park; Go Suzuki; Masanori Matsushita; Ill Sup Nou; Akira Isogai; Seiji Takayama; Masao Watanabe

doi:10.1266/ggs.79.87

Duplicated SP11 genes produce alternative transcripts in the S¹⁵ haplotype of Brassica oleracea

Hiroshi Shiba, Jong In Park, Go Suzuki, Masanori Matsushita, Ill Sup Nou, Akira Isogai, Seiji Takayama, Masao Watanabe

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S¹⁵-SP11a, S¹⁵-SP11b, and S¹⁵-SP11b', in the self-incompatible S ¹⁵ haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S¹⁵-SP11a (an exon-1 region of S ¹⁵-SP11b was also cotranscribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S¹⁵-SP11a, S ¹⁵-SP11b, and an exon-1 region of S¹⁵-SP11b', all three of which were connected to intergenic spacer regions.

Original language	English
Pages (from-to)	87-93
Number of pages	7
Journal	Genes and Genetic Systems
Volume	79
Issue number	2
DOIs	https://doi.org/10.1266/ggs.79.87
Publication status	Published - 2004 Apr
Externally published	Yes

Keywords

Alternative transcript
Brassica oleracea
Gene duplication
S locus protein 11 (SP11)
Self-incompatibility (SI)

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1266/ggs.79.87

Cite this

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title = "Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea",

abstract = "Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S 15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S 15-SP11b was also cotranscribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S 15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.",

keywords = "Alternative transcript, Brassica oleracea, Gene duplication, S locus protein 11 (SP11), Self-incompatibility (SI)",

author = "Hiroshi Shiba and Park, {Jong In} and Go Suzuki and Masanori Matsushita and Nou, {Ill Sup} and Akira Isogai and Seiji Takayama and Masao Watanabe",

year = "2004",

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doi = "10.1266/ggs.79.87",

language = "English",

volume = "79",

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T1 - Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea

AU - Shiba, Hiroshi

AU - Park, Jong In

AU - Suzuki, Go

AU - Matsushita, Masanori

AU - Nou, Ill Sup

AU - Isogai, Akira

AU - Takayama, Seiji

AU - Watanabe, Masao

PY - 2004/4

Y1 - 2004/4

N2 - Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S 15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S 15-SP11b was also cotranscribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S 15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.

AB - Self-incompatibility (SI) discriminating self and non-self pollen is regulated by S-locus genes in Brassica. In most S haplotypes, a set of three highly polymorphic genes, SLG, SRK, and SP11, is located at the S-locus region. In the present study, we found duplicated SP11 genes, S15-SP11a, S15-SP11b, and S15-SP11b', in the self-incompatible S 15 haplotype of B. oleracea. RNA gel blot and reverse transcription polymerase chain reaction (RT-PCR) analyses showed that two different sizes of SP11 transcripts were specifically detected in anther tissues: a 0.65-kb transcript corresponded to S15-SP11a (an exon-1 region of S 15-SP11b was also cotranscribed in some cases), and a 1.4-kb transcript contained the duplicated three genes, S15-SP11a, S 15-SP11b, and an exon-1 region of S15-SP11b', all three of which were connected to intergenic spacer regions.

KW - Alternative transcript

KW - Brassica oleracea

KW - Gene duplication

KW - S locus protein 11 (SP11)

KW - Self-incompatibility (SI)

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