Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision

Siew Cheng Phua, Shuhei Chiba, Masako Suzuki, Emily Su, Elle C. Roberson, Ganesh V. Pusapati, Mitsutoshi Setou, Rajat Rohatgi, Jeremy F. Reiter, Koji Ikegami, Takanari Inoue

Research output: Contribution to journalArticlepeer-review

225 Citations (Scopus)


The life cycle of a primary cilium begins in quiescence and ends prior to mitosis. In quiescent cells, the primary cilium insulates itself from contiguous dynamic membrane processes on the cell surface to function as a stable signaling apparatus. Here, we demonstrate that basal restriction of ciliary structure dynamics is established by the cilia-enriched phosphoinositide 5-phosphatase, Inpp5e. Growth induction displaces ciliary Inpp5e and accumulates phosphatidylinositol 4,5-bisphosphate in distal cilia. This change triggers otherwise-forbidden actin polymerization in primary cilia, which excises cilia tips in a process we call cilia decapitation. While cilia disassembly is traditionally thought to occur solely through resorption, we show that an acute loss of IFT-B through cilia decapitation precedes resorption. Finally, we propose that cilia decapitation induces mitogenic signaling and constitutes a molecular link between the cilia life cycle and cell-division cycle. This newly defined ciliary mechanism may find significance in cell proliferation control during normal development and cancer.

Original languageEnglish
Pages (from-to)264-279.e15
Issue number1-2
Publication statusPublished - 2017 Jan 12


  • AurA
  • F-actin
  • Gli
  • Inpp5e
  • PI(4,5)P
  • Primary cilia
  • cell-cycle entry
  • decapitation
  • disassembly
  • ectosome
  • extracellular vesicles
  • genetically encoded ciliary actin inhibitor


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