TY - JOUR
T1 - DzMab-1
T2 - Anti-Human Diacylglycerol Kinaseζ Monoclonal Antibody for Immunocytochemistry
AU - Nakano, Tomoyuki
AU - Ogasawara, Satoshi
AU - Tanaka, Toshiaki
AU - Hozumi, Yasukazu
AU - Sano, Masato
AU - Sayama, Yusuke
AU - Yamada, Shinji
AU - Kaneko, Mika K.
AU - Kato, Yukinari
AU - Goto, Kaoru
N1 - Funding Information:
K.G. and Y.K. received research funding from Ono Pharmaceutical Co., Ltd. The other authors have no conflicts of interest.
Funding Information:
This work was supported in part by AMED under Grant Number JP18am0101078 (Y.K.) and by the Grant-in-Aid for Challenging Exploratory Research from MEXT (T.N., K.G.).
Publisher Copyright:
© Copyright 2019, Mary Ann Liebert, Inc., publishers 2019.
PY - 2019/8
Y1 - 2019/8
N2 - Diacylglycerol kinase (DGK) is an enzyme that converts diacylglycerol (DG) to phosphatidic acid (PA). As both DG and PA serve as lipidic second messengers, DGK plays a pivotal role in controlling the balance of two signaling pathways mediated by DG and PA in cellular functions. DGKζ, one member of the mammalian DGK family, is reported to contain a nuclear localization signal, which suggests its functional role in the nucleus. Previously, morphological studies using tagged expression vectors and immunostaining of rat tissues or cells have revealed that DGKζ localizes mainly to the nucleus. However, a limited number of studies reported the detailed localization of native protein of DGKζ in human tissues and cells. In this study, we developed a novel anti-human DGKζ monoclonal antibody, DzMab-1, which is very advantageous in immunocytochemistry of human cultured cells.
AB - Diacylglycerol kinase (DGK) is an enzyme that converts diacylglycerol (DG) to phosphatidic acid (PA). As both DG and PA serve as lipidic second messengers, DGK plays a pivotal role in controlling the balance of two signaling pathways mediated by DG and PA in cellular functions. DGKζ, one member of the mammalian DGK family, is reported to contain a nuclear localization signal, which suggests its functional role in the nucleus. Previously, morphological studies using tagged expression vectors and immunostaining of rat tissues or cells have revealed that DGKζ localizes mainly to the nucleus. However, a limited number of studies reported the detailed localization of native protein of DGKζ in human tissues and cells. In this study, we developed a novel anti-human DGKζ monoclonal antibody, DzMab-1, which is very advantageous in immunocytochemistry of human cultured cells.
KW - DGKζ
KW - immunocytochemical analysis
KW - monoclonal antibody
UR - http://www.scopus.com/inward/record.url?scp=85070653843&partnerID=8YFLogxK
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U2 - 10.1089/mab.2019.0024
DO - 10.1089/mab.2019.0024
M3 - Article
C2 - 31403910
AN - SCOPUS:85070653843
SN - 2167-9436
VL - 38
SP - 179
EP - 182
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
IS - 4
ER -