TY - GEN
T1 - Ebpl expression is induced by influenza virus infection
AU - Ejima, Miho
AU - Ueda, Ryuta
AU - Kume, Shinichiro
AU - Okazaki, Daisuke
AU - Yamakawa, Takefumi
AU - Shiku, Hitoshi
AU - Honda, Ayae
PY - 2008
Y1 - 2008
N2 - Influenza virus PB1 protein plays the key role for the assembly and catalytic function of viral RNA polymerase. We showed that Ebpl interacted with PB1 and interfered both the viral RNA polymerase function in vitro and the virus growth. The Ebpl expression was induced by influenza virus infection using Western blotting and quantitative real time PCR assay. However, the quantitative determination has been requested of the relationship between influenza virus growth and Ebpl level. To analyze the relationship between virus growth and Ebpl level, we developed the assay system of single cell. Using this system, we confirmed the tight correlation between the Ebpl level and the influenza virus growth.
AB - Influenza virus PB1 protein plays the key role for the assembly and catalytic function of viral RNA polymerase. We showed that Ebpl interacted with PB1 and interfered both the viral RNA polymerase function in vitro and the virus growth. The Ebpl expression was induced by influenza virus infection using Western blotting and quantitative real time PCR assay. However, the quantitative determination has been requested of the relationship between influenza virus growth and Ebpl level. To analyze the relationship between virus growth and Ebpl level, we developed the assay system of single cell. Using this system, we confirmed the tight correlation between the Ebpl level and the influenza virus growth.
UR - http://www.scopus.com/inward/record.url?scp=62449087047&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=62449087047&partnerID=8YFLogxK
U2 - 10.1109/MHS.2008.4752452
DO - 10.1109/MHS.2008.4752452
M3 - Conference contribution
AN - SCOPUS:62449087047
SN - 9781424429196
T3 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008
SP - 214
EP - 218
BT - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics"
T2 - 2008 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2008, with Symposium on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "System Cell Engineering by Multi-scale Manipulation"
Y2 - 6 November 2008 through 9 November 2008
ER -