Economical Wet extraction of lipid from labyrinthula aura ntiochytrium limacinum by using liquefied dimethyl ether

Rintaro Hoshino; Kazuya Murakami; Siti Machmudah; Yuji Okita; Eiji Ohashi; Hideki Kanda; Motonobu Goto

Economical Wet extraction of lipid from labyrinthula aura ntiochytrium limacinum by using liquefied dimethyl ether

Rintaro Hoshino, Kazuya Murakami, Wahyudiono, Siti Machmudah, Yuji Okita, Eiji Ohashi, Hideki Kanda, Motonobu Goto

Research output: Contribution to conference › Paper › peer-review

Abstract

Recently, a simple method for the extraction of lipids from wet microalgae using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Herein, the versatility of this method was evaluated using Aurantiochytrium limacinum at 0.51 MPa and 20°C. Direct extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, in a lipid extraction yield of 46.1 wt% of the dry weight of the sample. On the other hands, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% by supercritical carbon dioxide (SCCO₂) extraction, Hexane-Soxhlet extraction and Bligh-Dyer (BD) extraction as conventional method. However, SCCO₂, hexane and BD methods, which incorporates drying and cell-disruption. The carbon and hydrogen content were improved after DME extraction. In addition, 86.7 wt% of water in the wet A. limacinum was removed. Compared with other extraction methods, liquefied DME combines drying, cell-disruption, and solvent extraction into one step.

Original language	English
Pages	372-380
Number of pages	9
Publication status	Published - 2015
Externally published	Yes
Event	13th International Symposium on East Asian Resources Recycling Technology, EARTH 2015 - Pattaya, Thailand Duration: 2015 Nov 1 → 2015 Nov 4

Other

Other	13th International Symposium on East Asian Resources Recycling Technology, EARTH 2015
Country/Territory	Thailand
City	Pattaya
Period	15/11/1 → 15/11/4

Keywords

Aurantiochytrium limacinum
Bio fuel
Dewatering
Fatty acid
Liquefied dimethyl ether
Supercritical carbon dioxide

ASJC Scopus subject areas

Environmental Science(all)

Cite this

Economical Wet extraction of lipid from labyrinthula aura ntiochytrium limacinum by using liquefied dimethyl ether. / Hoshino, Rintaro; Murakami, Kazuya; Wahyudiono et al.

2015. 372-380 Paper presented at 13th International Symposium on East Asian Resources Recycling Technology, EARTH 2015, Pattaya, Thailand.

Research output: Contribution to conference › Paper › peer-review

Hoshino, R, Murakami, K, Wahyudiono, Machmudah, S, Okita, Y, Ohashi, E, Kanda, H & Goto, M 2015, 'Economical Wet extraction of lipid from labyrinthula aura ntiochytrium limacinum by using liquefied dimethyl ether', Paper presented at 13th International Symposium on East Asian Resources Recycling Technology, EARTH 2015, Pattaya, Thailand, 15/11/1 - 15/11/4 pp. 372-380.

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abstract = "Recently, a simple method for the extraction of lipids from wet microalgae using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Herein, the versatility of this method was evaluated using Aurantiochytrium limacinum at 0.51 MPa and 20°C. Direct extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, in a lipid extraction yield of 46.1 wt% of the dry weight of the sample. On the other hands, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% by supercritical carbon dioxide (SCCO2) extraction, Hexane-Soxhlet extraction and Bligh-Dyer (BD) extraction as conventional method. However, SCCO2, hexane and BD methods, which incorporates drying and cell-disruption. The carbon and hydrogen content were improved after DME extraction. In addition, 86.7 wt% of water in the wet A. limacinum was removed. Compared with other extraction methods, liquefied DME combines drying, cell-disruption, and solvent extraction into one step.",

keywords = "Aurantiochytrium limacinum, Bio fuel, Dewatering, Fatty acid, Liquefied dimethyl ether, Supercritical carbon dioxide",

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T1 - Economical Wet extraction of lipid from labyrinthula aura ntiochytrium limacinum by using liquefied dimethyl ether

AU - Hoshino, Rintaro

AU - Murakami, Kazuya

AU - Wahyudiono,

AU - Machmudah, Siti

AU - Okita, Yuji

AU - Ohashi, Eiji

AU - Kanda, Hideki

AU - Goto, Motonobu

PY - 2015

Y1 - 2015

N2 - Recently, a simple method for the extraction of lipids from wet microalgae using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Herein, the versatility of this method was evaluated using Aurantiochytrium limacinum at 0.51 MPa and 20°C. Direct extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, in a lipid extraction yield of 46.1 wt% of the dry weight of the sample. On the other hands, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% by supercritical carbon dioxide (SCCO2) extraction, Hexane-Soxhlet extraction and Bligh-Dyer (BD) extraction as conventional method. However, SCCO2, hexane and BD methods, which incorporates drying and cell-disruption. The carbon and hydrogen content were improved after DME extraction. In addition, 86.7 wt% of water in the wet A. limacinum was removed. Compared with other extraction methods, liquefied DME combines drying, cell-disruption, and solvent extraction into one step.

AB - Recently, a simple method for the extraction of lipids from wet microalgae using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Herein, the versatility of this method was evaluated using Aurantiochytrium limacinum at 0.51 MPa and 20°C. Direct extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, in a lipid extraction yield of 46.1 wt% of the dry weight of the sample. On the other hands, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% by supercritical carbon dioxide (SCCO2) extraction, Hexane-Soxhlet extraction and Bligh-Dyer (BD) extraction as conventional method. However, SCCO2, hexane and BD methods, which incorporates drying and cell-disruption. The carbon and hydrogen content were improved after DME extraction. In addition, 86.7 wt% of water in the wet A. limacinum was removed. Compared with other extraction methods, liquefied DME combines drying, cell-disruption, and solvent extraction into one step.

KW - Aurantiochytrium limacinum

KW - Bio fuel

KW - Dewatering

KW - Fatty acid

KW - Liquefied dimethyl ether

KW - Supercritical carbon dioxide

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T2 - 13th International Symposium on East Asian Resources Recycling Technology, EARTH 2015

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ER -

Economical Wet extraction of lipid from labyrinthula aura ntiochytrium limacinum by using liquefied dimethyl ether

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ASJC Scopus subject areas

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