Abstract
In order to investigate the regulatory mechanisms of estrogen receptors (ER) in bone cells, changes in ER-alpha mRNA levels of rat osteosarcoma cell line (ROS 17/2.8) before and after exposure to 1,25(OH)2D3 and 17-beta estradiol respectively were measured by quantitative polymerase chain reaction using an internal standard. ER mRNA levels in the ROS 17/2.8 cultured with the medium alone had 5.029 ±1.623 mol/g total RNA x 10-13 and were not statistically different from those cultured in the presence of 1,25(OH)2D3 at concentrations of 10-12 M and less. ER mRNA levels in the ROS 17/2.8 cell line showed a small but a significant increase as a result of stimulation by 1,25(OH)2D3 at concentrations of 10-10 and 10-11 M. However, ER mRNA levels in ROS 17/2.8 cultured in the presence of 1,25(OH)2D3 at concentrations of 10-9 M were not statistically different from those of the control. On the other hand, the expression of ER in ROS 17/2.8 cells cultured for 3 hours with various doses of 1,25(OH)2D3 showed, by immunoblotting methods, a significant increase at the dose of 10-10 M in the expression of ER. Although a physiological significance is obscure, these observations suggest that 1,25(OH)2D3 plays a part in the expression of ER in ROS 17/2.8. No significant changes were seen in the expression of ER mRNA and the synthesis of ER as a result of stimulation by the estradiol.
| Original language | English |
|---|---|
| Pages (from-to) | 2465-2472 |
| Number of pages | 8 |
| Journal | Life Sciences |
| Volume | 66 |
| Issue number | 25 |
| DOIs | |
| Publication status | Published - 2000 May 12 |
| Externally published | Yes |
Keywords
- 1,25(OH)D
- ER mRNA
- Estrogen
- Estrogen receptors
- Quantitative PCR
- ROS 17/2.8
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)