TY - JOUR
T1 - Effect of calcium phosphate phases affecting the crosstalk between osteoblasts and osteoclasts in vitro
AU - Shiwaku, Yukari
AU - Tsuchiya, Kaori
AU - Xiao, Linghao
AU - Suzuki, Osamu
N1 - Funding Information:
Correspondence to: Osamu Suzuki; e-mail: suzuki-o@m.tohoku.ac.jp Contract grant sponsor: Grants-in-Aids for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (MEXT); contract grant number: 23106010, 17K19740, 18H02981, and 16K20482
Funding Information:
This study was supported in part by Grants-in-aids (23106010, 17K19740, 18H02981, and 16K20482) from the Ministry of Education, Science, Sports and Culture of Japan (MEXT). The authors are very grateful to Dr. Takamichi Miyazaki (School of Engineering, Tohoku University) for analysis of TEM. We are also grateful to Dr. Ryo Hamai for insightful comments. This study is partially supported by Tohoku University Center for Gender Equality Promotion (TUMUG) Support Project (Project to Promote Gender Equality and Female Researchers).
Publisher Copyright:
© 2019 Wiley Periodicals, Inc.
PY - 2019/5
Y1 - 2019/5
N2 - Previous studies have reported that octacalcium phosphate (OCP) enhances osteoblast differentiation and osteoclast formation during the hydrolysis process to hydroxyapatite (HA). However, the crystal phases that affect the crosstalk between osteoclasts and osteoblasts are unknown, which should determine the bone substitute material's property of OCP. The present study was designed to investigate whether the chemical composition and crystal structure of calcium phosphates affect osteoclast formation and the osteoclast–osteoblast crosstalk. Biodegradable β-tricalcium phosphate (β-TCP) was used as the control material. Osteoclasts were cultured on HA/OCP or HA/TCP disks and their cellular responses were assessed. Both OCP and β-TCP had a similar ability to create multinucleated osteoclasts. However, OCP promoted the expression of complement component 3a (C3a), a positive coupling factor, in osteoclasts, whereas β-TCP enhanced that of EphrinB2 (EfnB2) and collagen triple helix repeat containing 1 (Cthrc1). During osteoclast culture, phosphate ions were released from the crystals, and OCP-HA conversion was advanced in HA/OCP mixtures and OCP. X-ray diffraction analysis revealed no remarkable changes in the crystal structures of HA/TCP mixtures and β-TCP before and after osteoclast culture. These results indicate that the distinct chemical environment induced by the calcium phosphate phases affects the crosstalk between osteoclasts and osteoblasts.
AB - Previous studies have reported that octacalcium phosphate (OCP) enhances osteoblast differentiation and osteoclast formation during the hydrolysis process to hydroxyapatite (HA). However, the crystal phases that affect the crosstalk between osteoclasts and osteoblasts are unknown, which should determine the bone substitute material's property of OCP. The present study was designed to investigate whether the chemical composition and crystal structure of calcium phosphates affect osteoclast formation and the osteoclast–osteoblast crosstalk. Biodegradable β-tricalcium phosphate (β-TCP) was used as the control material. Osteoclasts were cultured on HA/OCP or HA/TCP disks and their cellular responses were assessed. Both OCP and β-TCP had a similar ability to create multinucleated osteoclasts. However, OCP promoted the expression of complement component 3a (C3a), a positive coupling factor, in osteoclasts, whereas β-TCP enhanced that of EphrinB2 (EfnB2) and collagen triple helix repeat containing 1 (Cthrc1). During osteoclast culture, phosphate ions were released from the crystals, and OCP-HA conversion was advanced in HA/OCP mixtures and OCP. X-ray diffraction analysis revealed no remarkable changes in the crystal structures of HA/TCP mixtures and β-TCP before and after osteoclast culture. These results indicate that the distinct chemical environment induced by the calcium phosphate phases affects the crosstalk between osteoclasts and osteoblasts.
KW - hydroxyapatite
KW - octacalcium phosphate
KW - osteoclast–osteoblast crosstalk
KW - β-tricalcium phosphate
UR - http://www.scopus.com/inward/record.url?scp=85061429989&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85061429989&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.36626
DO - 10.1002/jbm.a.36626
M3 - Article
C2 - 30684383
AN - SCOPUS:85061429989
SN - 1549-3296
VL - 107
SP - 1001
EP - 1013
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
IS - 5
ER -