TY - JOUR
T1 - Effect of dwell time on carbonyl stress using icodextrin and amino acid peritoneal dialysis fluids
AU - Ueda, Yasuhiko
AU - Miyata, Toshio
AU - Goffin, Eric
AU - Yoshino, Atsushi
AU - Inagi, Reiko
AU - Ishibashi, Yoshitaka
AU - Izuhara, Yuko
AU - Saito, Akira
AU - Kurokawa, Kiyoshi
AU - Van Ypersele de Strihou, Charles
N1 - Funding Information:
This study was supported by grants from Research for the Future Program of the Japan Society for the Promotion of Science (96L00303), from the Japanese Ministry of Health and Welfare for Research on Health Services (H10-079), and from the Baxter Healthcare Corporation.
PY - 2000/12
Y1 - 2000/12
N2 - Background. Deterioration of the peritoneal membrane limits the technical survival of peritoneal dialysis (PD). Advanced glycation of the membrane has been incriminated in this evolution. Advanced glycation end products (AGEs) develop under the influence of glucose and of its degradation products, mainly reactive carbonyl compounds (RCOs) such as glyoxal (GO), methylglyoxal (MGO), and 3-deoxyglucosone (3-DG). The present study was undertaken to evaluate the impact of recently developed glucose-free PD fluids on AGE generation. Methods. Recently developed glucose-free PD fluids containing either icodextrin or amino acids were investigated. GO, MGO, and 3-DG [high-performance liquid chromatography (HPLC)] and total RCOs (spectrophotometry) were measured in fresh solutions and in effluents after various dwell duration. The AGE formation potential of PD fluids and effluents was assessed by incubation at 37°C, for one week, with bovine serum albumin and by the eventual measurement of pentosidine (HPLC) and N(ε)-carboxymethyllysine (CML; gas chromatography/mass spectrometry). Results. GO, MGO, and 3-DG (P < 0.001) as well as total RCOs levels (P < 0.01) were significantly lower in icodextrin and amino acid PD fluid than in commercial, heat-sterilized, 1.36 % glucose PD fluid. Pentosidine and CML generation were also significantly lower (P < 0.001) in icodextrin and amino acid PD fluid than in conventional 1.36% glucose PD fluid. The levels of total RCOs, however, increased in icodextrin and amino acid PD fluid effluents with dwell time. AGE formation potential rose accordingly, as demonstrated by a parallel increase in the generation of pentosidine and CML during incubation of PD effluents. Conclusion. The present data demonstrate lower RCO contents and AGE formation potential in fresh icodextrin and amino acid PD fluids than in fresh heat-sterilized glucose PD fluids. However, this difference decreases progressively during dwell time, mainly as a result of the influx of total RCOs.
AB - Background. Deterioration of the peritoneal membrane limits the technical survival of peritoneal dialysis (PD). Advanced glycation of the membrane has been incriminated in this evolution. Advanced glycation end products (AGEs) develop under the influence of glucose and of its degradation products, mainly reactive carbonyl compounds (RCOs) such as glyoxal (GO), methylglyoxal (MGO), and 3-deoxyglucosone (3-DG). The present study was undertaken to evaluate the impact of recently developed glucose-free PD fluids on AGE generation. Methods. Recently developed glucose-free PD fluids containing either icodextrin or amino acids were investigated. GO, MGO, and 3-DG [high-performance liquid chromatography (HPLC)] and total RCOs (spectrophotometry) were measured in fresh solutions and in effluents after various dwell duration. The AGE formation potential of PD fluids and effluents was assessed by incubation at 37°C, for one week, with bovine serum albumin and by the eventual measurement of pentosidine (HPLC) and N(ε)-carboxymethyllysine (CML; gas chromatography/mass spectrometry). Results. GO, MGO, and 3-DG (P < 0.001) as well as total RCOs levels (P < 0.01) were significantly lower in icodextrin and amino acid PD fluid than in commercial, heat-sterilized, 1.36 % glucose PD fluid. Pentosidine and CML generation were also significantly lower (P < 0.001) in icodextrin and amino acid PD fluid than in conventional 1.36% glucose PD fluid. The levels of total RCOs, however, increased in icodextrin and amino acid PD fluid effluents with dwell time. AGE formation potential rose accordingly, as demonstrated by a parallel increase in the generation of pentosidine and CML during incubation of PD effluents. Conclusion. The present data demonstrate lower RCO contents and AGE formation potential in fresh icodextrin and amino acid PD fluids than in fresh heat-sterilized glucose PD fluids. However, this difference decreases progressively during dwell time, mainly as a result of the influx of total RCOs.
KW - Advanced glycation end product
KW - Biocompatibility
KW - Glucose degradation product
KW - Peritoneal dialysis fluids
KW - Reactive carbonyl compound
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U2 - 10.1046/j.1523-1755.2000.00436.x
DO - 10.1046/j.1523-1755.2000.00436.x
M3 - Article
C2 - 11115086
AN - SCOPUS:0033666283
SN - 0085-2538
VL - 58
SP - 2518
EP - 2524
JO - Kidney International
JF - Kidney International
IS - 6
ER -