TY - JOUR
T1 - Effect of hyaluronan to inhibit caspase activation in porcine granulosa cells
AU - Tunjung, Woro Anindito Sri
AU - Yokoo, Masaki
AU - Hoshino, Yumi
AU - Miyake, Yuko
AU - Kadowaki, Akane
AU - Sato, Eimei
N1 - Funding Information:
This work was supported by a grant from the Japan Society for the Promotion of Science JSPS-16108003 (to E.S.).
PY - 2009/4/24
Y1 - 2009/4/24
N2 - We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus-oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA, expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44 antibody decreased the expression of procaspases suggesting that perturbation of HA-CD44 binding leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA-CD44 binding is important on apoptosis inhibitory mechanism in porcine granulosa cells.
AB - We studied the ability of hyaluronan (HA) to inhibit apoptosis in porcine granulosa cells. The granulosa layer with cumulus-oocyte complex is cultured in media supplemented with follicle stimulating hormone (FSH) and 4-MU an inhibitor of hyaluronan synthases. The concentration of HA significantly increased after supplemented with FSH, but significantly decreased with 4-MU. CD44, receptor of HA, expressed after cultured with FSH, decreased in addition low concentration of 4-MU, whereas not detected in high concentration of 4-MU, indicating parallel relation between the amount of HA and CD44 expression. The 4-MU treatment also decreased the expression of procaspase-3, -8, -9 suggesting that inhibition of HA synthesis leads to activation of these caspases. Moreover, addition of anti-CD44 antibody decreased the expression of procaspases suggesting that perturbation of HA-CD44 binding leads activation of caspases. Hence, HA has ability to inhibit apoptosis and HA-CD44 binding is important on apoptosis inhibitory mechanism in porcine granulosa cells.
KW - Apoptosis of granulosa cells
KW - CD44
KW - Caspase
KW - FSH
KW - Hyaluronan
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U2 - 10.1016/j.bbrc.2009.02.163
DO - 10.1016/j.bbrc.2009.02.163
M3 - Article
C2 - 19268653
AN - SCOPUS:63149179115
SN - 0006-291X
VL - 382
SP - 160
EP - 164
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -