Effect of intraluminal pressure on the intimal thickening in injured rabbit carotid arteries in an organ-culture system

Hisanori Horiuchi; Masayuki Yokode; Kenji Ishii; Mikimaro Cho; Seijiro Hara; Atsushi Sakai; Kazuo Kanai; Toyohiro Tsukada; Toru Kita

doi:10.1253/jcj.61.704

Effect of intraluminal pressure on the intimal thickening in injured rabbit carotid arteries in an organ-culture system

Hisanori Horiuchi, Masayuki Yokode, Kenji Ishii, Mikimaro Cho, Seijiro Hara, Atsushi Sakai, Kazuo Kanai, Toyohiro Tsukada, Toru Kita

IDAC - Division of Aging Science

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Abstract

We developed an organ culture system in order to examine the effect of intraluminal pressure on intimal thickening in injured arteries. After endothelial denudation, an excised rabbit common carotid artery was incubated at 37°C in a glass bottle perfused with Dulbecco's modified Eagle medium containing 10% fetal calf serum at a constant flow rate of 6 ml/h at various static pressure of 60, 95, 130, 165, or 200 cmH₂O. Intimal thickening, which consisted of smooth muscle cells and extracellular matrix, increased in a time- and pressure-dependent manner up to 130 cmH₂O. Excessive pressures (165 and 200 cmH₂O), however, caused necrosis in the media. These results suggest that a rise in intraluminal pressure accelerates intimal thickening in injured arteries.

Original language	English
Pages (from-to)	704-708
Number of pages	5
Journal	Japanese Circulation Journal
Volume	61
Issue number	8
DOIs	https://doi.org/10.1253/jcj.61.704
Publication status	Published - 1997

Keywords

Hypertension
Injured artery
Intimal thickening
Organ culture
Stretching

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10.1253/jcj.61.704

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title = "Effect of intraluminal pressure on the intimal thickening in injured rabbit carotid arteries in an organ-culture system",

abstract = "We developed an organ culture system in order to examine the effect of intraluminal pressure on intimal thickening in injured arteries. After endothelial denudation, an excised rabbit common carotid artery was incubated at 37°C in a glass bottle perfused with Dulbecco's modified Eagle medium containing 10% fetal calf serum at a constant flow rate of 6 ml/h at various static pressure of 60, 95, 130, 165, or 200 cmH2O. Intimal thickening, which consisted of smooth muscle cells and extracellular matrix, increased in a time- and pressure-dependent manner up to 130 cmH2O. Excessive pressures (165 and 200 cmH2O), however, caused necrosis in the media. These results suggest that a rise in intraluminal pressure accelerates intimal thickening in injured arteries.",

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AU - Horiuchi, Hisanori

AU - Yokode, Masayuki

AU - Ishii, Kenji

AU - Cho, Mikimaro

AU - Hara, Seijiro

AU - Sakai, Atsushi

AU - Kanai, Kazuo

AU - Tsukada, Toyohiro

AU - Kita, Toru

PY - 1997

Y1 - 1997

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AB - We developed an organ culture system in order to examine the effect of intraluminal pressure on intimal thickening in injured arteries. After endothelial denudation, an excised rabbit common carotid artery was incubated at 37°C in a glass bottle perfused with Dulbecco's modified Eagle medium containing 10% fetal calf serum at a constant flow rate of 6 ml/h at various static pressure of 60, 95, 130, 165, or 200 cmH2O. Intimal thickening, which consisted of smooth muscle cells and extracellular matrix, increased in a time- and pressure-dependent manner up to 130 cmH2O. Excessive pressures (165 and 200 cmH2O), however, caused necrosis in the media. These results suggest that a rise in intraluminal pressure accelerates intimal thickening in injured arteries.

KW - Hypertension

KW - Injured artery

KW - Intimal thickening

KW - Organ culture

KW - Stretching

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ER -

Effect of intraluminal pressure on the intimal thickening in injured rabbit carotid arteries in an organ-culture system

Abstract

Keywords

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