Effect of Mg 2+ on neural activity of rat cortical and hippocampal neurons in vitro

Yuriko Furukawa, Nahoko Kasai, Keiichi Torimitsu

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24 Citations (Scopus)


Mg 2+ plays an important role in biological functions, similar to that of Ca 2+. In terms of neural activity, it is well known that Mg 2+ blocks the NMDA receptor. However, the relationship between Mg 2+ and neural function has not been well understood. We have investigated the effect of low extracellular Mg 2+ concentration ([Mg 2+] o) on neural activity in rat cortical and hippocampal neurons by using microelectrode array (MEA) measurements and glutamate measurements, with an enzyme modified MEA-based multi-array sensor. In this study, we investigated the effects of low [Mg 2+] o on intracellular Ca 2+ concentration ([Ca 2+] i) using a confocal laser microscope and a flow cytometer with a fluorescence probe. The results indicate that low [Mg 2+] o has an effect on neural activity. The responses of cortical and hippocampal neurons to low [Mg 2+] o differed in the developmental period. The results suggest that hippocampal neurons are more sensitive to [Mg 2+] o than cortical neurons. The glutamate receptor distributions in the cortex and hippocampus may be different. Further investigation is required to understand the mechanisms of the Mg 2+ effect on neural activity.

Original languageEnglish
Pages (from-to)174-181
Number of pages8
JournalMagnesium Research
Issue number3
Publication statusPublished - 2009 Sept


  • Ca
  • Cortex
  • Flow cytometry
  • Hippocampus
  • MEA
  • Mg


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