TY - JOUR
T1 - Effect of nano-hydroxyapatite on bone morphogenetic protein-2-induced hard tissue formation and dentin resorption on a dentin surface
AU - Tamagawa, Hiroki
AU - Tenkumo, Taichi
AU - Sugaya, Tsutomu
AU - Kawanami, Masamitsu
N1 - Funding Information:
This work is financially supported by Grants-in-Aid for Scientific Research (No. 22791917 ) from Japan Society for the Promotion of Science . We thank Dr. Gelinsky for information concerning preparation of the mineralized collagen membranes and Dr. Nakazawa and Dr. Nishio for their encouragement throughout the course of this work.
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Aim: The purpose of this study was to evaluate the effects of the addition of nano-hydroxyapatite to a collagen membrane-carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) on hard tissue formation and dentin resorption on dentin surfaces in vivo. Materials and methods: Nano-hydroxyapatite collagen composite (nHAC) membranes or collagen (C) membranes were each immersed in either 100 or 400 μg/ml rhBMP-2 and placed on dentin chips that were implanted into rat thigh muscle. The implants were analyzed at 2 or 4 weeks after surgery by histological observation and histomorphometric analysis. Results: The percentage of the hard tissue formed by each nHAC group was significantly higher than that formed by any of the C groups, except for that formed by the group loaded with 400 μg/ml rhBMP-2 at 4 weeks after implantation. No significant differences were observed in the percentage of dentin resorption between the nHAC groups and C groups at any stage or at any rhBMP-2 concentration. Conclusion: These findings showed that addition of nano-hydroxyapatite to a collagen membrane accelerated the formation of hard tissue induced by a low dose of rhBMP-2 on dentin surfaces at an early stage after implantation into rat thigh muscle, without increasing dentin resorption.
AB - Aim: The purpose of this study was to evaluate the effects of the addition of nano-hydroxyapatite to a collagen membrane-carrier of recombinant human bone morphogenetic protein-2 (rhBMP-2) on hard tissue formation and dentin resorption on dentin surfaces in vivo. Materials and methods: Nano-hydroxyapatite collagen composite (nHAC) membranes or collagen (C) membranes were each immersed in either 100 or 400 μg/ml rhBMP-2 and placed on dentin chips that were implanted into rat thigh muscle. The implants were analyzed at 2 or 4 weeks after surgery by histological observation and histomorphometric analysis. Results: The percentage of the hard tissue formed by each nHAC group was significantly higher than that formed by any of the C groups, except for that formed by the group loaded with 400 μg/ml rhBMP-2 at 4 weeks after implantation. No significant differences were observed in the percentage of dentin resorption between the nHAC groups and C groups at any stage or at any rhBMP-2 concentration. Conclusion: These findings showed that addition of nano-hydroxyapatite to a collagen membrane accelerated the formation of hard tissue induced by a low dose of rhBMP-2 on dentin surfaces at an early stage after implantation into rat thigh muscle, without increasing dentin resorption.
KW - Dentin resorption
KW - Hard tissue formation
KW - Nano-hydroxyapatite
KW - rhBMP-2
UR - http://www.scopus.com/inward/record.url?scp=84869090162&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84869090162&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2012.03.103
DO - 10.1016/j.apsusc.2012.03.103
M3 - Article
AN - SCOPUS:84869090162
SN - 0169-4332
VL - 262
SP - 140
EP - 145
JO - Applied Surface Science
JF - Applied Surface Science
ER -