Effective concentration of ionic liquids for enhanced saccharification of cellulose

Kazuhiko Tanimura, Yoshiko Ooe, Keishi Suga, Hiroshi Umakoshi

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)


In an aqueous enzymatic saccharification using cellulase, the dissolution of crystalline cellulose is one of the rate-limiting steps. Insoluble cellulose powder was preliminarily heat-treated with ionic liquids (ILs), such as [Bmim][Cl] (1-butyl-3-methylimidazolium chloride) and [Amim][Cl] (1-allyl-3-methylimidazolium chloride), which enable the production of soluble cellulose. On the other hand, the presence of ILs leads to a denaturation of enzymes. Using cellulase from Trichoderma viride, the effects of [Bmim][Cl] and [Amim][Cl] in the enzymatic saccharification were compared. The production of glucose was optimized with 5 wt%-ILs, both for [Bmim][Cl] and for [Amim][Cl]. The significant inhibiting effects of ILs (IL concentration >10 wt%) could be due to the denaturation of cellulase, because the peak shifts of intrinsic tryptophan fluorescence were observed in the presence of 7.5 wt%-ILs. To analyze kinetic parameters, the Langmuir adsorption model and the Michaelis-Menten model were employed. The investigation suggests that [Amim][Cl] can provide soluble cellulose more efficiently, and can promote enzymatic saccharification in the IL concentration below 5 wt%.

Original languageEnglish
Article number47
Pages (from-to)1-8
Number of pages8
Issue number4
Publication statusPublished - 2018 Dec
Externally publishedYes


  • Cellulase
  • Hydrolysis
  • Insoluble cellulose
  • Ionic liquid
  • Kinetic analysis

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Engineering(all)
  • Energy(all)


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