Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor

Wakako Ikeda-Ohtsubo; Morio Miyahara; Takeshi Yamada; Akira Watanabe; Shinya Fushinobu; Takayoshi Wakagi; Hirofumi Shoun; Keisuke Miyauchi; Ginro Endo

doi:10.1016/j.jbiosc.2013.05.024

Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor

Wakako Ikeda-Ohtsubo, Morio Miyahara, Takeshi Yamada, Akira Watanabe, Shinya Fushinobu, Takayoshi Wakagi, Hirofumi Shoun, Keisuke Miyauchi, Ginro Endo

AGR - Agricultural Bioscience

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Bioaugmentation of bioreactor systems with pre-cultured bacteria has proven difficult because inoculated bacteria are easily eliminated by predatory eukaryotic-microorganisms. Here, we demonstrated an intermediate thermal treatment was effective for protecting introduced denitrifying bacteria from eukaryotic predators and consequently allowed the inoculated bacteria to survive longer in a denitrification reactor.

Original language	English
Pages (from-to)	722-724
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	116
Issue number	6
DOIs	https://doi.org/10.1016/j.jbiosc.2013.05.024
Publication status	Published - 2013 Dec

Keywords

18S rRNA genes
Bioaugmentation
Denitrifying bacteria
Heat treatment
Predatory protists

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10.1016/j.jbiosc.2013.05.024

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Ikeda-Ohtsubo, W., Miyahara, M., Yamada, T., Watanabe, A., Fushinobu, S., Wakagi, T., Shoun, H., Miyauchi, K., & Endo, G. (2013). Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor. Journal of Bioscience and Bioengineering, 116(6), 722-724. https://doi.org/10.1016/j.jbiosc.2013.05.024

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title = "Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor",

abstract = "Bioaugmentation of bioreactor systems with pre-cultured bacteria has proven difficult because inoculated bacteria are easily eliminated by predatory eukaryotic-microorganisms. Here, we demonstrated an intermediate thermal treatment was effective for protecting introduced denitrifying bacteria from eukaryotic predators and consequently allowed the inoculated bacteria to survive longer in a denitrification reactor.",

keywords = "18S rRNA genes, Bioaugmentation, Denitrifying bacteria, Heat treatment, Predatory protists",

author = "Wakako Ikeda-Ohtsubo and Morio Miyahara and Takeshi Yamada and Akira Watanabe and Shinya Fushinobu and Takayoshi Wakagi and Hirofumi Shoun and Keisuke Miyauchi and Ginro Endo",

note = "Funding Information: This study was supported by Grant-in-Aids for Scientific Research from the Japan Society for the Promotion of Science (nos. 20248009 and 21360258 ). We thank Simon Silver (UIC) for providing useful comments and advice. We also thank Kanji Nakamura for his technical advice throughout this study. ",

year = "2013",

month = dec,

doi = "10.1016/j.jbiosc.2013.05.024",

language = "English",

volume = "116",

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journal = "Journal of Bioscience and Bioengineering",

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Ikeda-Ohtsubo, W, Miyahara, M, Yamada, T, Watanabe, A, Fushinobu, S, Wakagi, T, Shoun, H, Miyauchi, K & Endo, G 2013, 'Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor', Journal of Bioscience and Bioengineering, vol. 116, no. 6, pp. 722-724. https://doi.org/10.1016/j.jbiosc.2013.05.024

TY - JOUR

T1 - Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor

AU - Ikeda-Ohtsubo, Wakako

AU - Miyahara, Morio

AU - Yamada, Takeshi

AU - Watanabe, Akira

AU - Fushinobu, Shinya

AU - Wakagi, Takayoshi

AU - Shoun, Hirofumi

AU - Miyauchi, Keisuke

AU - Endo, Ginro

N1 - Funding Information: This study was supported by Grant-in-Aids for Scientific Research from the Japan Society for the Promotion of Science (nos. 20248009 and 21360258 ). We thank Simon Silver (UIC) for providing useful comments and advice. We also thank Kanji Nakamura for his technical advice throughout this study.

PY - 2013/12

Y1 - 2013/12

N2 - Bioaugmentation of bioreactor systems with pre-cultured bacteria has proven difficult because inoculated bacteria are easily eliminated by predatory eukaryotic-microorganisms. Here, we demonstrated an intermediate thermal treatment was effective for protecting introduced denitrifying bacteria from eukaryotic predators and consequently allowed the inoculated bacteria to survive longer in a denitrification reactor.

AB - Bioaugmentation of bioreactor systems with pre-cultured bacteria has proven difficult because inoculated bacteria are easily eliminated by predatory eukaryotic-microorganisms. Here, we demonstrated an intermediate thermal treatment was effective for protecting introduced denitrifying bacteria from eukaryotic predators and consequently allowed the inoculated bacteria to survive longer in a denitrification reactor.

KW - 18S rRNA genes

KW - Bioaugmentation

KW - Denitrifying bacteria

KW - Heat treatment

KW - Predatory protists

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DO - 10.1016/j.jbiosc.2013.05.024

M3 - Article

C2 - 23810658

AN - SCOPUS:84886405481

SN - 1389-1723

VL - 116

SP - 722

EP - 724

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

IS - 6

ER -

Effectiveness of heat treatment to protect introduced denitrifying bacteria from eukaryotic predatory microorganisms in a pilot-scale bioreactor

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Keywords

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