TY - JOUR
T1 - Effects of a Novel Piezo Actuator-driven Pulsed Water Jet System on Residual Kidney After Partial Nephrectomy in a Rat Model
AU - Kamiyama, Yoshihiro
AU - Yamashita, Shinichi
AU - Nakagawa, Atsuhiro
AU - Fujii, Shinji
AU - Goto, Takuro
AU - Mitsuzuka, Koji
AU - Ito, Akihiro
AU - Abe, Takaaki
AU - Tominaga, Teiji
AU - Arai, Yoichi
N1 - Funding Information:
Funding Support: This work was supported in part by the Translational Research Network Program from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT), the Japanese Foundation for Research and Promotion of Endoscopy Grant, Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science(15H01707, 18H04157, 26282116, 15H04945, 26462196, 26462197, 24592049, 26461297, 15K10353, 15K10572), the Miyagi Prefecture Kidney Association Grant, and the Clinical Research Promotion Program for Young Investigators of Tohoku University Hospital.
Funding Information:
AN and TT received research support for this study from Seiko Epson Co., Ltd. under a collaborative research contract with Tohoku University.
Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2019/1
Y1 - 2019/1
N2 - Objective: To evaluate renal damage after off-clamp partial nephrectomy (PN) using a novel surgical device, piezo actuator-driven pulsed water jet (ADPJ) system. Methods: Sprague-Dawley rats were divided into 4 groups and subjected to sham operation, off-clamp PN by the piezo ADPJ system, radio knife, and soft coagulation, which have been used as thermal coagulation devices. Urine and blood samples were collected, and residual kidneys were harvested at 1, 7, 14, 30, and 90 days after PN. Serum blood urea nitrogen, creatinine, and urinary and serum kidney injury molecule-1 KIM-1 levels were measured. Morphological features and the extent of renal ischemia of resection surfaces were evaluated by hematoxylin-eosin staining and immunostaining using antibodies to 1-methyladenosine, respectively. In addition, the expression levels of KIM-1 mRNA extracted from each resection surface were analyzed by quantitative real-time reverse transcription polymerase chain reaction. Results: Serum blood urea nitrogen and creatinine were significantly lower with the piezo ADPJ system than with soft coagulation. Urinary and serum KIM-1 levels were also significantly decreased with the ADPJ. The extent of 1-methyladenosine immunostaining was significantly less with the ADPJ than with thermal coagulation devices. The expression levels of KIM-1 mRNA were also lower in the ADPJ system group. Conclusion: The piezo ADPJ system might attenuate renal damage after off-clamp PN.
AB - Objective: To evaluate renal damage after off-clamp partial nephrectomy (PN) using a novel surgical device, piezo actuator-driven pulsed water jet (ADPJ) system. Methods: Sprague-Dawley rats were divided into 4 groups and subjected to sham operation, off-clamp PN by the piezo ADPJ system, radio knife, and soft coagulation, which have been used as thermal coagulation devices. Urine and blood samples were collected, and residual kidneys were harvested at 1, 7, 14, 30, and 90 days after PN. Serum blood urea nitrogen, creatinine, and urinary and serum kidney injury molecule-1 KIM-1 levels were measured. Morphological features and the extent of renal ischemia of resection surfaces were evaluated by hematoxylin-eosin staining and immunostaining using antibodies to 1-methyladenosine, respectively. In addition, the expression levels of KIM-1 mRNA extracted from each resection surface were analyzed by quantitative real-time reverse transcription polymerase chain reaction. Results: Serum blood urea nitrogen and creatinine were significantly lower with the piezo ADPJ system than with soft coagulation. Urinary and serum KIM-1 levels were also significantly decreased with the ADPJ. The extent of 1-methyladenosine immunostaining was significantly less with the ADPJ than with thermal coagulation devices. The expression levels of KIM-1 mRNA were also lower in the ADPJ system group. Conclusion: The piezo ADPJ system might attenuate renal damage after off-clamp PN.
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U2 - 10.1016/j.urology.2018.10.014
DO - 10.1016/j.urology.2018.10.014
M3 - Article
C2 - 30359707
AN - SCOPUS:85056992954
SN - 0090-4295
VL - 123
SP - 265
EP - 272
JO - Urology
JF - Urology
ER -