TY - JOUR
T1 - Effects of co-overproduction of Rubisco and chloroplast glyceraldehyde-3-phosphate dehydrogenase on photosynthesis in rice
AU - Suzuki, Yuji
AU - Ishiyama, Keiki
AU - Cho, Ayaka
AU - Takegahara-Tamakawa, Yuki
AU - Wada, Shinya
AU - Miyake, Chikahiro
AU - Makino, Amane
N1 - Funding Information:
This study was supported by Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency (Grant No. JPMJCR15O3 to Y.S. and C.M.) and Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 18H02111 to Y.S. and No. 16H06379 to A.M.). We would like to thank Dr. Uzuki Matsushima (Iwate University) for the aid in measurements of photosynthesis, and Editage for the English editing.
Funding Information:
This work was supported by Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency [JPMJCR15O3]; Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science [16H06379,18H02111]. This study was supported by Core Research for Evolutional Science and Technology from the Japan Science and Technology Agency (Grant No. JPMJCR15O3 to Y.S. and C.M.) and Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (No. 18H02111 to Y.S. and No. 16H06379 to A.M.). We would like to thank Dr. Uzuki Matsushima (Iwate University) for the aid in measurements of photosynthesis, and Editage for the English editing.
Publisher Copyright:
© 2021 Japanese Society of Soil Science and Plant Nutrition.
PY - 2021
Y1 - 2021
N2 - The capacity for regeneration of the substrate for Rubisco, ribulose 1,5-bisphosphate (RuBP), is one of the candidates for the bottleneck in photosynthesis in transgenic rice plants with overproduction of Rubisco. As the overproduction of chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was suggested to slightly enhance RuBP regeneration capacity, transgenic rice plants with co-overproduction of Rubisco and GAPDH were generated to improve photosynthesis. The amount of Rubisco increased by 8% to 18%, whereas GAPDH activity increased to 2.1- to 3.2-fold that of the wild-type. However, the rate of CO2 assimilation did not increase under conditions of high irradiance and different [CO2] partial pressures. The possibility that photosynthesis in the co-overproduced plants was co-limited by Rubisco, GAPDH, and other unknown factors is discussed.
AB - The capacity for regeneration of the substrate for Rubisco, ribulose 1,5-bisphosphate (RuBP), is one of the candidates for the bottleneck in photosynthesis in transgenic rice plants with overproduction of Rubisco. As the overproduction of chloroplast glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was suggested to slightly enhance RuBP regeneration capacity, transgenic rice plants with co-overproduction of Rubisco and GAPDH were generated to improve photosynthesis. The amount of Rubisco increased by 8% to 18%, whereas GAPDH activity increased to 2.1- to 3.2-fold that of the wild-type. However, the rate of CO2 assimilation did not increase under conditions of high irradiance and different [CO2] partial pressures. The possibility that photosynthesis in the co-overproduced plants was co-limited by Rubisco, GAPDH, and other unknown factors is discussed.
KW - Chloroplast glyceraldehyde-3-phosphate dehydrogenase
KW - co-overproduction
KW - CO assimilation
KW - rice
KW - Rubisco
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U2 - 10.1080/00380768.2021.1915100
DO - 10.1080/00380768.2021.1915100
M3 - Article
AN - SCOPUS:85104691913
SN - 0038-0768
VL - 67
SP - 283
EP - 287
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
IS - 3
ER -