Effects of Cu2+ on conformational change and aggregation of hPrP180-192 with a V180I mutation of the prion protein

Yuko Sakaguchi, Rina Nakamura, Motomi Konishi, Yusuke Hatakawa, Hidenao Toyoda, Toshifumi Akizawa

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Prion diseases are neurodegenerative disorders caused by misfolding of the prion protein (PrP) from a normal cellular protein (PrPC) to a protease-resistant isoform (PrPSc). However, the aggregation mechanism is not entirely understood because of the physical properties of PrP, such as its solubility or aggregation in vitro and conformational or mutation diversity. Recently, we reported the physical and physiological properties of a synthetic fragment peptide. In the present study, we assessed the importance of a point mutation at the C-terminal region of PrP in structural conversion and aggregation and evaluated the physical and physiological properties of the point-mutated human-PrP180-192 V180I (hPrP180-192 V180I) using circular dichroism spectra, high-performance liquid chromatography, Affinix QNμ, and thioflavin-T staining, including the effects of Cu2+. The secondary structure of hPrP180-192 V180I changed from a random coil to a β-sheet in Cu2+ free buffer. In addition, we observed molecular interactions in hPrP180-192 V180I and aggregation with itself, which were inhibited by Cu2+. We conclude that the point mutation in the C-terminal region of PrP, including hPrP180-192 V180I, and Cu2+ may play an important role in the conversion of PrPC to PrPSc.

Original languageEnglish
Pages (from-to)798-802
Number of pages5
JournalBiochemical and biophysical research communications
Issue number3
Publication statusPublished - 2019 Jun 30
Externally publishedYes


  • Aggregation
  • Circular dichroism spectra
  • Cu
  • HPLC analysis
  • Intermolecular interaction
  • Point-mutated hPrP180-192
  • Prion protein
  • Structural conversion
  • Thioflavin-T

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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