Effects of diuretics on calcium uptake and release in renal microsomes

Dan Takashi, Gemba Munekazu

Research output: Contribution to journalArticlepeer-review


A variety of diuretics were tested for their effects on Ca2+ uptake and release by microsomes isolated from rat kidneys. Mersalyl acid, ethacrynic acid, furosemide and bumetanide inhibited Ca2+ uptake by microsomes. Cysteine abolished the inhibitory effects of mersalyl acid and ethacrynic acid on Ca2+ uptake. No appreciable differences in the inhibition were seen between microsomes from kidney cortex and those from medulla. Acetazolamide had no significant effect on Ca2+ uptake; however. hydrochlorothiazide stimulated Ca2+ uptake activity. The effects of the diuretics on Ca2+ uptake by microsomes were entirely on the process of Ca2+ accumulation in microsomes, since release of Ca2+ that had been taken up previously was not influenced by any of the diuretics tested. These results provide information concerning the biochemical mechanisms of action of diuretics on Ca2+ reabsorption in the kidney.

Original languageEnglish
Pages (from-to)2339-2343
Number of pages5
JournalBiochemical Pharmacology
Issue number17
Publication statusPublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology


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