TY - JOUR
T1 - Effects of ethanol and its metabolites on human pancreatic stellate cells
AU - Masamune, Atsushi
AU - Satoh, Akihiko
AU - Watanabe, Takashi
AU - Kikuta, Kazuhiro
AU - Satoh, Masahiro
AU - Suzuki, Noriaki
AU - Satoh, Kennichi
AU - Shimosegawa, Tooru
N1 - Funding Information:
Acknowledgments This research was supported in part by a Grant-in-Aid from the Japan Society for the Promotion of Science (to A.M. and to A.S.), by the Pancreas Research Foundation of Japan (to A.M. and to K.K.), by the Kanae Foundation for Life and Socio-Medical Science (to A.M.), and by the Uehara Memorial Foundation (to A.M.).
PY - 2010/1
Y1 - 2010/1
N2 - Pancreatic stellate cells (PSCs) play a pivotal role in pancreatic inflammation and fibrosis. In the pancreas, in addition to oxidative metabolism, ethanol can be metabolized by esterification with fatty acids to form fatty acid ethyl esters such as palmitic acid ethyl ester (PAEE). We here examined the effects of ethanol (at 20 or 50 mM), acetaldehyde (at 200 μM), or PAEE (at 100 μM), on PSCs functions. PSCs did not express mRNAs for pancreatic triglyceride lipase and carboxyl ester lipase. Ethanol and acetaldehyde, but not PAEE, induced production of procollagen type I C-peptide. Ethanol, but not acetaldehyde or PAEE, induced interleukin-8 production. PAEE activated activator protein-1, but not nuclear factor κB. In addition, PAEE activated extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase. Specific activation of signal transduction pathways and cell functions by ethanol and its metabolites may play a role in alcohol-induced pancreatic injury.
AB - Pancreatic stellate cells (PSCs) play a pivotal role in pancreatic inflammation and fibrosis. In the pancreas, in addition to oxidative metabolism, ethanol can be metabolized by esterification with fatty acids to form fatty acid ethyl esters such as palmitic acid ethyl ester (PAEE). We here examined the effects of ethanol (at 20 or 50 mM), acetaldehyde (at 200 μM), or PAEE (at 100 μM), on PSCs functions. PSCs did not express mRNAs for pancreatic triglyceride lipase and carboxyl ester lipase. Ethanol and acetaldehyde, but not PAEE, induced production of procollagen type I C-peptide. Ethanol, but not acetaldehyde or PAEE, induced interleukin-8 production. PAEE activated activator protein-1, but not nuclear factor κB. In addition, PAEE activated extracellular signal-regulated kinase, c-Jun N-terminal kinase, and p38 mitogen-activated protein kinase. Specific activation of signal transduction pathways and cell functions by ethanol and its metabolites may play a role in alcohol-induced pancreatic injury.
KW - Acetaldehyde
KW - Fatty acid ethyl ester
KW - Pancreatic fibrosis
KW - Pancreatitis
KW - Signal transduction
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U2 - 10.1007/s10620-008-0695-y
DO - 10.1007/s10620-008-0695-y
M3 - Article
C2 - 19165599
AN - SCOPUS:74049098550
SN - 0163-2116
VL - 55
SP - 204
EP - 211
JO - Digestive Diseases and Sciences
JF - Digestive Diseases and Sciences
IS - 1
ER -