TY - JOUR
T1 - Effects of exercise training on renal interstitial fibrosis and renin-angiotensin system in rats with chronic renal failure
AU - Yamakoshi, Seiko
AU - Nakamura, Takahiro
AU - Mori, Nobuyoshi
AU - Suda, Chihiro
AU - Kohzuki, Masahiro
AU - Ito, Osamu
N1 - Funding Information:
We thank the Institute for Animal Experimentation of Tohoku University Graduate School of Medicine for animal care, and the Biomedical Research Unit of Tohoku University Hospital and the Biomedical Research Core of Tohoku University Graduate School of Medicine for technical assistance. The current study was supported in part by a grant from the Ministry of Education, Culture, Sports, Science and Technology (16H03199, 17H02119, 17H02118).
Publisher Copyright:
© 2021 Lippincott Williams and Wilkins. All rights reserved.
PY - 2021/1
Y1 - 2021/1
N2 - Objective: To clarify the mechanisms of the renal protective effects of exercise training, we examined the effects of exercise training on the renal interstitial fibrosis and renin-angiotensin system (RAS) in rats with chronic renal failure. Methods: Six-week-old male Sprague-Dawley rats were divided into three groups: Sham operation; 5/6 nephrectomyRsedentary; 5/6 nephrectomyRexercise training. The 5/6 nephrectomyRexercise training group underwent treadmill running (20 m/min, 60 min/day, 5 days/week). After 12 weeks, renal function, histology and protein expression of collagen type I, transforming growth factor-b1 (TGF-b1), matrix metalloproteinase (MMP), tissue inhibitors of metalloproteinase (TIMP) and RAS components in the renal cortex were examined. Results: Exercise training ameliorated the 5/6 nephrectomy-induced hypertension, proteinuria, renal dysfunction, glomerular sclerosis and renal interstitial fibrosis. 5/6 Nephrectomy increased the expression of collagen type I, TGF-b1, MMP-2, MMP-9, TIMP-1, angiotensinogen, angiotensin-converting enzyme (ACE), (pro)renin receptor and angiotensin II type 1 receptor, and exercise training inhibited the 5/6 nephrectomy-increased expression of collagen type I, TGF-b1, TIMP-1, angiotensinogen and ACE expressions. 5/6 Nephrectomy decreased the expression of renin, ACE2, angiotensin II type 2 receptor and Mas receptor, and exercise training inhibited the 5/6 nephrectomy-decreased expressions. Conclusion: These results indicated that exercise training attenuates the progression of glomerular sclerosis and renal interstitial fibrosis in chronic renal failure rats. The renal protective effects of exercise training may be mediated by ameliorating the renal collagen turnover and the exacerbation of renal RAS.
AB - Objective: To clarify the mechanisms of the renal protective effects of exercise training, we examined the effects of exercise training on the renal interstitial fibrosis and renin-angiotensin system (RAS) in rats with chronic renal failure. Methods: Six-week-old male Sprague-Dawley rats were divided into three groups: Sham operation; 5/6 nephrectomyRsedentary; 5/6 nephrectomyRexercise training. The 5/6 nephrectomyRexercise training group underwent treadmill running (20 m/min, 60 min/day, 5 days/week). After 12 weeks, renal function, histology and protein expression of collagen type I, transforming growth factor-b1 (TGF-b1), matrix metalloproteinase (MMP), tissue inhibitors of metalloproteinase (TIMP) and RAS components in the renal cortex were examined. Results: Exercise training ameliorated the 5/6 nephrectomy-induced hypertension, proteinuria, renal dysfunction, glomerular sclerosis and renal interstitial fibrosis. 5/6 Nephrectomy increased the expression of collagen type I, TGF-b1, MMP-2, MMP-9, TIMP-1, angiotensinogen, angiotensin-converting enzyme (ACE), (pro)renin receptor and angiotensin II type 1 receptor, and exercise training inhibited the 5/6 nephrectomy-increased expression of collagen type I, TGF-b1, TIMP-1, angiotensinogen and ACE expressions. 5/6 Nephrectomy decreased the expression of renin, ACE2, angiotensin II type 2 receptor and Mas receptor, and exercise training inhibited the 5/6 nephrectomy-decreased expressions. Conclusion: These results indicated that exercise training attenuates the progression of glomerular sclerosis and renal interstitial fibrosis in chronic renal failure rats. The renal protective effects of exercise training may be mediated by ameliorating the renal collagen turnover and the exacerbation of renal RAS.
KW - Chronic renal failure
KW - Collagen metabolism
KW - Exercise
KW - Renal interstitial fibrosis
KW - Renin-angiotensin system
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U2 - 10.1097/HJH.0000000000002605
DO - 10.1097/HJH.0000000000002605
M3 - Article
C2 - 32833922
AN - SCOPUS:85097211188
SN - 0263-6352
VL - 39
SP - 143
EP - 152
JO - Journal of Hypertension
JF - Journal of Hypertension
IS - 1
ER -