TY - JOUR
T1 - Effects of food lectins on the transport system of human intestinal CaCO-2 cell monolayers
AU - Yamamoto, Shintaro
AU - Tomiyama, Mai
AU - Nemoto, Ryo
AU - Naganuma, Takako
AU - Ogawa, Tomohisa
AU - Muramoto, Koji
N1 - Funding Information:
This work was supported by JSPS KAKENHI grant no. 23380121 and funding by the Ministry of Agriculture, Forestry and Fisheries of the Japanese Government.
PY - 2013
Y1 - 2013
N2 - The effects of 16 lectins isolated from foodstuff on the transport system across human intestinal Caco-2 cell monolayers were investigated by using four fluorescent markers: lucifer yellow (LY) for the paracellular pathway, fluorescein (FL) for the monocarboxylic acid transporter-mediated pathway, rhodamine 123 for the P-glycoprotein-mediated efflux pathway, and calcein for the multidrug resistance associated protein-related efflux pathway. The transepithelial electrical resistance (TER) values for the monolayers were also measured. WGA from wheat germ, ABA from white mushroom, AOL from Aspergillus oryzae, and CSL3 from chum salmon eggs (each at 100μg/mL) decreased the TER value by 20-40% which resulted in increased LY transport. These lectins, as well as such other lectins as SBA from soybean, RBA from rice bran, and Con A from jack bean, affected other transport pathways too. These results indicate that the lectins modulated the transepithelial transport system in different ways, probably because of their specific binding characteristics toward Caco-2 cell monolayers.
AB - The effects of 16 lectins isolated from foodstuff on the transport system across human intestinal Caco-2 cell monolayers were investigated by using four fluorescent markers: lucifer yellow (LY) for the paracellular pathway, fluorescein (FL) for the monocarboxylic acid transporter-mediated pathway, rhodamine 123 for the P-glycoprotein-mediated efflux pathway, and calcein for the multidrug resistance associated protein-related efflux pathway. The transepithelial electrical resistance (TER) values for the monolayers were also measured. WGA from wheat germ, ABA from white mushroom, AOL from Aspergillus oryzae, and CSL3 from chum salmon eggs (each at 100μg/mL) decreased the TER value by 20-40% which resulted in increased LY transport. These lectins, as well as such other lectins as SBA from soybean, RBA from rice bran, and Con A from jack bean, affected other transport pathways too. These results indicate that the lectins modulated the transepithelial transport system in different ways, probably because of their specific binding characteristics toward Caco-2 cell monolayers.
KW - Caco-2 cell
KW - Intestinal transport
KW - Lectin
KW - Tight junction
KW - Transepithelial transport
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U2 - 10.1271/bbb.130367
DO - 10.1271/bbb.130367
M3 - Article
C2 - 24018688
AN - SCOPUS:84884640970
SN - 0916-8451
VL - 77
SP - 1917
EP - 1924
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
IS - 9
ER -