Effects of L-NAME and L-arginine on diaphragm contraction in a septic animal model

C. Shindoh, D. Wu, Y. Ohuchi, H. Kurosawa, Y. Kikuchi, W. Hida, K. Shirato

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8 Citations (Scopus)


The effects of nitric oxide on diaphragm contraction after endotoxin administration were studied in Wistar rats. The animals were divided into seven treatments: a saline-injected group as control, three groups injected with L-NAME (0.01, 0.1, 1 mg/kg) and three groups injected with L-arginine (1, 10, 100 mg/kg). Escherichia coli endotoxin was injected into the peritoneal cavity 15 min later. Twitch kinetics and force-frequency curves were measured 0, 2, and 4 hr after endotoxin injection. In the control group, the force-frequency curves significantly decreased from 0 hr to 4 hr. In the L-NAME group, the force-frequency curves at 4 hr showed significant increases in a dose-dependent manner. In the L-arginine group, the force-frequency curve with 100 mg/kg at 4 hr showed a significant increase. There was no consistent change in the contraction time, half relaxation time, or fatiguability. NADPH diaphorase histochemistry performed on diaphragm muscle samples 4 hr after endotoxin injection showed positive in the control and L- arginine group, but was only weakly observed in L-NAME group. These data suggest that nitric oxide contributes to the endotoxin induced diaphragm contractile deterioration.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalComparative biochemistry and physiology. Part A, Molecular & integrative physiology
Issue number1
Publication statusPublished - 1998 Jan


  • Endotoxin
  • Force- frequency curves
  • Free radicals
  • Muscle fatigue
  • NADPH diaphorase
  • Nitric oxide
  • Superoxide
  • Twitch kinetics


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