TY - JOUR
T1 - Effects of Long-Term Exercise on Liver Cyst in Polycystic Liver Disease Model Rats
AU - Sato, Yoichi
AU - Qiu, Jiahe
AU - Miura, Takahiro
AU - Kohzuki, Masahiro
AU - Ito, Osamu
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (JSPS KAKENHI nos. 15K12573 and 17H02119). The authors greatly appreciate technical support from the Biomedical Research Unit of Tohoku University Hospital in histopathological analysis.
Publisher Copyright:
Copyright © 2019 The Author(s).
PY - 2020/6/1
Y1 - 2020/6/1
N2 - Background: Polycystic liver disease (PLD) is a hereditary liver disease with progressive enlargement of fluid-filled liver cysts, which causes abdominal discomfort and worsens quality of life. Long-term exercise has beneficial effects in various organs, but the effects of long-term exercise on PLD are unclear. Therefore, the aim of this study was to investigate whether long-term exercise inhibits liver cyst formation and fibrosis. Methods: Polycystic kidney (PCK) rats, a model of PLD, were randomly divided into a sedentary group and a long-term exercise group, which underwent treadmill running for 12 wk (28 m·min−1, 60 min·d−1, 5 d·wk−1). Sprague-Dawley (SD) rats were set as a control group. After 12 wk, exercise capacity, histology, and signaling cascades of PLD were examined. Results: Compared with control SD rats, PCK rats showed a low exercise capacity before exercise protocol. After 12 wk, the exercise improved the exercise capacity and ameliorated liver cyst formation and fibrosis. The exercise significantly decreased the number of Ki-67-positive cells; the expression of cystic fibrosis transmembrane conductance regulator, aquaporin 1, transforming growth factor β, and type 1 collagen; and the phosphorylation of extracellular signal-regulated kinase, mammalian target of rapamycin and S6. It also increased the phosphorylation of AMP-activated protein kinase in the liver of PCK rats. Conclusions: The present results indicated that long-term moderate-intensity exercise ameliorates liver cyst formation and fibrosis with the inhibition of signaling cascades responsible for cellular proliferation and fibrosis in PCK rats.
AB - Background: Polycystic liver disease (PLD) is a hereditary liver disease with progressive enlargement of fluid-filled liver cysts, which causes abdominal discomfort and worsens quality of life. Long-term exercise has beneficial effects in various organs, but the effects of long-term exercise on PLD are unclear. Therefore, the aim of this study was to investigate whether long-term exercise inhibits liver cyst formation and fibrosis. Methods: Polycystic kidney (PCK) rats, a model of PLD, were randomly divided into a sedentary group and a long-term exercise group, which underwent treadmill running for 12 wk (28 m·min−1, 60 min·d−1, 5 d·wk−1). Sprague-Dawley (SD) rats were set as a control group. After 12 wk, exercise capacity, histology, and signaling cascades of PLD were examined. Results: Compared with control SD rats, PCK rats showed a low exercise capacity before exercise protocol. After 12 wk, the exercise improved the exercise capacity and ameliorated liver cyst formation and fibrosis. The exercise significantly decreased the number of Ki-67-positive cells; the expression of cystic fibrosis transmembrane conductance regulator, aquaporin 1, transforming growth factor β, and type 1 collagen; and the phosphorylation of extracellular signal-regulated kinase, mammalian target of rapamycin and S6. It also increased the phosphorylation of AMP-activated protein kinase in the liver of PCK rats. Conclusions: The present results indicated that long-term moderate-intensity exercise ameliorates liver cyst formation and fibrosis with the inhibition of signaling cascades responsible for cellular proliferation and fibrosis in PCK rats.
KW - CELLULAR PROLIFERATION
KW - EXERCISE
KW - FIBROSIS
KW - POLYCYSTIC LIVER DISEASE
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U2 - 10.1249/MSS.0000000000002251
DO - 10.1249/MSS.0000000000002251
M3 - Article
C2 - 31880641
AN - SCOPUS:85085229740
SN - 0195-9131
VL - 52
SP - 1272
EP - 1279
JO - Medicine and Science in Sports and Exercise
JF - Medicine and Science in Sports and Exercise
IS - 6
ER -