TY - JOUR
T1 - Effects of Low-Intensity Pulsed Ultrasound for Preventing Joint Stiffness in Immobilized Knee Model in Rats
AU - Itaya, Nobuyuki
AU - Yabe, Yutake
AU - Hagiwara, Yoshihiro
AU - Kanazawa, Kenji
AU - Koide, Masashi
AU - Sekiguchi, Takuya
AU - Yoshida, Shinichirou
AU - Sogi, Yasuhito
AU - Yano, Toshihisa
AU - Tsuchiya, Masahiro
AU - Saijo, Yoshihumi
AU - Itoi, Eiji
N1 - Publisher Copyright:
© 2018 World Federation for Ultrasound in Medicine and Biology
PY - 2018/6
Y1 - 2018/6
N2 - The purpose of this study was to examine the effect of low-intensity pulsed ultrasound (LIPUS) in preventing joint stiffness. Unilateral knee joints were immobilized in two groups of rats (n = 6/period/group). Under general anesthesia, the immobilized knee joints were exposed to LIPUS for 20 min/d, 5 d/wk, using an existing LIPUS device (LIPUS group, 1.5-MHz frequency, 1.0-kHz repetition cycle, 200-µs burst width and 30-mW/cm2 power output) until endpoints (2, 4 or 6 wk). In the control group, general anesthesia alone was administered in the same manner as in the other group. The variables compared between the groups included joint angles; histologic, histomorphometric and immunohistochemical analyses; quantitative reverse transcription polymerase chain reactions; and tissue elasticity. LIPUS had a preventive effect on joint stiffness, resulting in decreased adhesion, fibrosis and inflammation and hypoxic response after joint immobilization.
AB - The purpose of this study was to examine the effect of low-intensity pulsed ultrasound (LIPUS) in preventing joint stiffness. Unilateral knee joints were immobilized in two groups of rats (n = 6/period/group). Under general anesthesia, the immobilized knee joints were exposed to LIPUS for 20 min/d, 5 d/wk, using an existing LIPUS device (LIPUS group, 1.5-MHz frequency, 1.0-kHz repetition cycle, 200-µs burst width and 30-mW/cm2 power output) until endpoints (2, 4 or 6 wk). In the control group, general anesthesia alone was administered in the same manner as in the other group. The variables compared between the groups included joint angles; histologic, histomorphometric and immunohistochemical analyses; quantitative reverse transcription polymerase chain reactions; and tissue elasticity. LIPUS had a preventive effect on joint stiffness, resulting in decreased adhesion, fibrosis and inflammation and hypoxic response after joint immobilization.
KW - Adhesion
KW - Elasticity
KW - Fibrosis
KW - Hypoxia
KW - Inflammation
KW - Joint contracture
KW - Joint immobilization
KW - Joint stiffness
KW - LIPUS
KW - Low-intensity pulsed ultrasound
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U2 - 10.1016/j.ultrasmedbio.2018.02.002
DO - 10.1016/j.ultrasmedbio.2018.02.002
M3 - Article
C2 - 29573888
AN - SCOPUS:85044278941
SN - 0301-5629
VL - 44
SP - 1244
EP - 1256
JO - Ultrasound in Medicine and Biology
JF - Ultrasound in Medicine and Biology
IS - 6
ER -