TY - JOUR
T1 - Effects of mitomycin C and colchicine on toxin production and cell cycle regulation in the dinoflagellate Alexandrium tamarense
AU - Cho, Yuko
AU - Ogawa, Motoo
AU - Hirota, Mayumi
AU - Oshima, Yasukatsu
PY - 2011/3
Y1 - 2011/3
N2 - Paralytic shellfish toxin (PST) production and cell proliferation in the dinoflagellate Alexandrium tamarense were investigated under the influence of two metabolic inhibitors (mitomycin C and colchicine) that have different mechanisms of action. Intracellular PST levels in cells treated with 2μM mitomycin C increased gradually, reaching a maximum of 176fmol/cell (a 6-fold increase). High concentrations of colchicine prolonged G1 phase in A. tamarense cells, even though colchicine arrests several other eukaryotic cell types in M phase. The cells in prolonged G1 phase under the influence of colchicine were apparently unable to produce PST. Cell proliferation and toxin production recovered after removal of colchicine; in contrast, the effect of mitomycin C was irreversible. While the timing of toxin production within the cell cycle was not conclusively determined, A. tamarense cells in S phase were able to produce PST.
AB - Paralytic shellfish toxin (PST) production and cell proliferation in the dinoflagellate Alexandrium tamarense were investigated under the influence of two metabolic inhibitors (mitomycin C and colchicine) that have different mechanisms of action. Intracellular PST levels in cells treated with 2μM mitomycin C increased gradually, reaching a maximum of 176fmol/cell (a 6-fold increase). High concentrations of colchicine prolonged G1 phase in A. tamarense cells, even though colchicine arrests several other eukaryotic cell types in M phase. The cells in prolonged G1 phase under the influence of colchicine were apparently unable to produce PST. Cell proliferation and toxin production recovered after removal of colchicine; in contrast, the effect of mitomycin C was irreversible. While the timing of toxin production within the cell cycle was not conclusively determined, A. tamarense cells in S phase were able to produce PST.
KW - Alexandrium tamarense
KW - Colchicine
KW - Mitomycin C
KW - Paralytic shellfish toxins
UR - http://www.scopus.com/inward/record.url?scp=79451476117&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79451476117&partnerID=8YFLogxK
U2 - 10.1016/j.hal.2010.10.003
DO - 10.1016/j.hal.2010.10.003
M3 - Article
AN - SCOPUS:79451476117
SN - 1568-9883
VL - 10
SP - 235
EP - 244
JO - Harmful Algae
JF - Harmful Algae
IS - 3
ER -