Effects of ozone nano-bubble water on periodontopathic bacteria and oral cells - In vitro studies

Sae Hayakumo, Shinichi Arakawa, Masayoshi Takahashi, Keiko Kondo, Yoshihiro Mano, Yuichi Izumi

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)


The aims of the present study were to evaluate the bactericidal activity of a new antiseptic agent, ozone nano-bubble water (NBW3), against periodontopathogenic bacteria and to assess the cytotoxicity of NBW3 against human oral cells. The bactericidal activities of NBW3 against representative periodontopathogenic bacteria, Porphyromonas gingivalis (P. gingivalis) and Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) were evaluated using in vitro time-kill assays. The cytotoxicity of NBW3 was evaluated using three-dimensional human buccal and gingival tissue models. The numbers of colony forming units (CFUs)/mL of P. gingivalis and A. actinomycetemcomitans exposed to NBW3 dropped to below the lower limit of detection (<10 CFUs mL-1) after only 0.5 min of exposure. There were only minor decreases in the viability of oral tissue cells after 24 h of exposure to NBW3. These results suggest that NBW3 possesses potent bactericidal activity against representative periodontopathogenic bacteria and is not cytotoxic to cells of human oral tissues. The use of NBW3 as an adjunct to periodontal therapy would be promising.

Original languageEnglish
Article number055003
JournalScience and Technology of Advanced Materials
Issue number5
Publication statusPublished - 2014 Oct 1
Externally publishedYes


  • antiseptic
  • cytotoxicity
  • ozone nano-bubble water
  • periodontitis
  • periodontopathic bacteria

ASJC Scopus subject areas

  • Materials Science(all)


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