TY - JOUR
T1 - Effects of seed sludge on fermentative characteristics and microbial community structures in thermophilic hydrogen fermentation of starch
AU - Akutsu, Yohei
AU - Li, Yu You
AU - Tandukar, Madan
AU - Kubota, Kengo
AU - Harada, Hideki
N1 - Funding Information:
This study was financially supported by the Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT), Japan Science and Technology Agency (JST), and the Japan Society for the Promotion of Science (JSPS). The first author was supported by JSPS research fellowship for young scientists.
PY - 2008/11
Y1 - 2008/11
N2 - In this work, effects of seed sludge on thermophilic hydrogen fermentation were investigated. Five different kinds of sludge were separately inoculated in completely stirred tank reactors (CSTRs), without any pretreatment. The reactors were operated in parallel with starch as substrate under thermophilic condition (55 °C) at a hydraulic retention time (HRT) of 24 h for 50 days. Stable hydrogen production was achieved in all reactors. The highest hydrogen yield (2.32 mol H2/mol glucose) was obtained from the reactor seeded with thermophilically digested activated sludge. The stoichiometric equation of hydrogen fermentation calculated using the data obtained in this study was expressed as follows: starch ([C6H10O5]n) → 2.32n H2 + 2.14n CO2 + 0.50n acetate + 0.63n butyrate + 0.11n cell (C5H7NO2). Microbial community structure was analyzed by polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE) and 16S rRNA gene cloning. Close relatives of the Thermoanaerobacterium were found to be the most predominated one in all reactors.
AB - In this work, effects of seed sludge on thermophilic hydrogen fermentation were investigated. Five different kinds of sludge were separately inoculated in completely stirred tank reactors (CSTRs), without any pretreatment. The reactors were operated in parallel with starch as substrate under thermophilic condition (55 °C) at a hydraulic retention time (HRT) of 24 h for 50 days. Stable hydrogen production was achieved in all reactors. The highest hydrogen yield (2.32 mol H2/mol glucose) was obtained from the reactor seeded with thermophilically digested activated sludge. The stoichiometric equation of hydrogen fermentation calculated using the data obtained in this study was expressed as follows: starch ([C6H10O5]n) → 2.32n H2 + 2.14n CO2 + 0.50n acetate + 0.63n butyrate + 0.11n cell (C5H7NO2). Microbial community structure was analyzed by polymerase chain reaction-denaturing gel gradient electrophoresis (PCR-DGGE) and 16S rRNA gene cloning. Close relatives of the Thermoanaerobacterium were found to be the most predominated one in all reactors.
KW - Microbial community structure
KW - Seed sludge
KW - Stoichiometric equation
KW - Thermoanaerobacterium
KW - Thermophilic hydrogen fermentation
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U2 - 10.1016/j.ijhydene.2008.08.038
DO - 10.1016/j.ijhydene.2008.08.038
M3 - Article
AN - SCOPUS:55149105873
SN - 0360-3199
VL - 33
SP - 6541
EP - 6548
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 22
ER -
