Efficient regeneration from GUS-transformed Ajuga hairy root

Nobuyuki Uozumi, Yasuki Ohtake, Yutaka Nakashimada, Yutaka Morikawa, Nobukazu Tanaka, Takeshi Kobayashi

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23 Citations (Scopus)


A procedure for the systemic regeneration from Ajuga hairy root was developed. Supplementation of an auxin, naphthaleneacetic acid (0.1 mg/l), in the hairy root growth culture enhanced the cell growh rate. Mechanical fragmentation for 10 s in a commercial blender enabled a large number of root fragments to be produced eficiently. Supplementation of a cytokinin, benzyladenine (10 mg/l), exhibited the highest plantlet formation frequency in the plantlet formation stage. The plantlets longer than 4 mm in length were able to grow into whole plants on agar containing modified MS medium. Ajuga hairy root in which the β-glucuronidase (GUS) gene was introduced under control of the tomato ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit (rbcS3B) promoter was constructed by the Agrobacterium rhizogenes-mediated co-traansformation. The GUS-transformed hairy root could also be efficiently regenerated into plantlets through this procedure. GUS activity was detected in leaf tissue of the regenerated plants.

Original languageEnglish
Pages (from-to)374-378
Number of pages5
JournalJournal of Fermentation and Bioengineering
Issue number5
Publication statusPublished - 1996


  • Ajuga
  • hairy root
  • micropropagation
  • regeneration
  • transgenic plant


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