Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels

Matsuhiko Nishizawa; Hirokazu Kaji; Kazuto Tsukidate; Masahiko Hashimoto; Takashi Abe

doi:10.1109/SENSOR.2005.1496381

Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels

Matsuhiko Nishizawa, Hirokazu Kaji, Kazuto Tsukidate, Masahiko Hashimoto, Takashi Abe

ENG - Finemechanics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Citation (Scopus)

Abstract

The use of microfluidic system is a recent trend in diagnostic assays and bioreactors based on the protein and cellular engineering. Most of microfluidic biodevices would require the combination with a technique to pattern proteins and living cells within microchannels just prior to the use of the devices, since these bio-elements are delicate against desiccation, oxidation and heat. Here, we present a simple, electrochemical method to locally immobilize proteins and cells on substrate surface, easily applicable to the microfluidic system. The integration of the technique into a microfluidic device and the on-demand immobilization of proteins and cells were achieved. The sandwich immunoassay within the microchannel was demonstrated, in which a series of protocols from immobilization to measurement can be carried out under physiochemical conditions.

Original language	English
Title of host publication	TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers
Pages	151-154
Number of pages	4
DOIs	https://doi.org/10.1109/SENSOR.2005.1496381
Publication status	Published - 2005 Nov 9
Event	13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05 - Seoul, Korea, Republic of Duration: 2005 Jun 5 → 2005 Jun 9

Publication series

Name	Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Volume	1

Other

Other	13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05
Country/Territory	Korea, Republic of
City	Seoul
Period	05/6/5 → 05/6/9

Keywords

Lithography
Microchannel
Microelectrode
Micropatterning
Protein adsorption

ASJC Scopus subject areas

Engineering(all)

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10.1109/SENSOR.2005.1496381

Cite this

Nishizawa, M., Kaji, H., Tsukidate, K., Hashimoto, M., & Abe, T. (2005). Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers (pp. 151-154). [1D4.3] (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05; Vol. 1). https://doi.org/10.1109/SENSOR.2005.1496381

Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels. / Nishizawa, Matsuhiko; Kaji, Hirokazu; Tsukidate, Kazuto et al.
TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. p. 151-154 1D4.3 (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05; Vol. 1).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Nishizawa, M, Kaji, H, Tsukidate, K, Hashimoto, M & Abe, T 2005, Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels. in TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers., 1D4.3, Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05, vol. 1, pp. 151-154, 13th International Conference on Solid-State Sensors and Actuators and Microsystems, TRANSDUCERS '05, Seoul, Korea, Republic of, 05/6/5. https://doi.org/10.1109/SENSOR.2005.1496381

Nishizawa M, Kaji H, Tsukidate K, Hashimoto M, Abe T. Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels. In TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. p. 151-154. 1D4.3. (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05). doi: 10.1109/SENSOR.2005.1496381

Nishizawa, Matsuhiko ; Kaji, Hirokazu ; Tsukidate, Kazuto et al. / Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels. TRANSDUCERS '05 - 13th International Conference on Solid-State Sensors and Actuators and Microsystems - Digest of Technical Papers. 2005. pp. 151-154 (Digest of Technical Papers - International Conference on Solid State Sensors and Actuators and Microsystems, TRANSDUCERS '05).

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AU - Tsukidate, Kazuto

AU - Hashimoto, Masahiko

AU - Abe, Takashi

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AB - The use of microfluidic system is a recent trend in diagnostic assays and bioreactors based on the protein and cellular engineering. Most of microfluidic biodevices would require the combination with a technique to pattern proteins and living cells within microchannels just prior to the use of the devices, since these bio-elements are delicate against desiccation, oxidation and heat. Here, we present a simple, electrochemical method to locally immobilize proteins and cells on substrate surface, easily applicable to the microfluidic system. The integration of the technique into a microfluidic device and the on-demand immobilization of proteins and cells were achieved. The sandwich immunoassay within the microchannel was demonstrated, in which a series of protocols from immobilization to measurement can be carried out under physiochemical conditions.

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Elcrochemical bio-lithography for in-situ immobilization of proteins and cells within microchannels

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