Electrical stimulation modulates fate determination of differentiating embryonic stem cells

Masahisa Yamada; Kentaro Tanemura; Seiji Okada; Akio Iwanami; Masaya Nakamura; Hideaki Mizuno; Michiru Ozawa; Ritsuko Ohyama-Goto; Naohito Kitamura; Masako Kawano; Kyoko Tan-Takeuchi; Chiho Ohtsuka; Atsushi Miyawaki; Akihiko Takashima; Masaharu Ogawa; Yoshiaki Toyama; Hideyuki Okano; Takashi Kondo

doi:10.1634/stemcells.2006-0011

Electrical stimulation modulates fate determination of differentiating embryonic stem cells

Masahisa Yamada, Kentaro Tanemura, Seiji Okada, Akio Iwanami, Masaya Nakamura, Hideaki Mizuno, Michiru Ozawa, Ritsuko Ohyama-Goto, Naohito Kitamura, Masako Kawano, Kyoko Tan-Takeuchi, Chiho Ohtsuka, Atsushi Miyawaki, Akihiko Takashima, Masaharu Ogawa, Yoshiaki Toyama, Hideyuki Okano, Takashi Kondo

Research output: Contribution to journal › Article › peer-review

158 Citations (Scopus)

Abstract

A clear understanding of cell fate regulation during differentiation is key in successfully using stem cells for therapeutic applications. Here, we report that mild electrical stimulation strongly influences embryonic stem cells to assume a neuronal fate. Although the resulting neuronal cells showed no sign of specific terminal differentiation in culture, they showed potential to differentiate into various types of neurons in vivo, and, in adult mice, contributed to the injured spinal cord as neuronal cells. Induction of calcium ion influx is significant in this differentiation system. This phenomenon opens up possibilities for understanding novel mechanisms underlying cellular differentiation and early development, and, perhaps more importantly, suggests possibilities for treatments in medical contexts.

Original language	English
Pages (from-to)	562-570
Number of pages	9
Journal	Stem Cells
Volume	25
Issue number	3
DOIs	https://doi.org/10.1634/stemcells.2006-0011
Publication status	Published - 2007 Mar
Externally published	Yes

Keywords

Calcium flux
Embryonic stem cells
Neuron
Tissue regeneration

ASJC Scopus subject areas

Medicine(all)

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10.1634/stemcells.2006-0011

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Yamada, M., Tanemura, K., Okada, S., Iwanami, A., Nakamura, M., Mizuno, H., Ozawa, M., Ohyama-Goto, R., Kitamura, N., Kawano, M., Tan-Takeuchi, K., Ohtsuka, C., Miyawaki, A., Takashima, A., Ogawa, M., Toyama, Y., Okano, H., & Kondo, T. (2007). Electrical stimulation modulates fate determination of differentiating embryonic stem cells. Stem Cells, 25(3), 562-570. https://doi.org/10.1634/stemcells.2006-0011

Yamada, M, Tanemura, K, Okada, S, Iwanami, A, Nakamura, M, Mizuno, H, Ozawa, M, Ohyama-Goto, R, Kitamura, N, Kawano, M, Tan-Takeuchi, K, Ohtsuka, C, Miyawaki, A, Takashima, A, Ogawa, M, Toyama, Y, Okano, H & Kondo, T 2007, 'Electrical stimulation modulates fate determination of differentiating embryonic stem cells', Stem Cells, vol. 25, no. 3, pp. 562-570. https://doi.org/10.1634/stemcells.2006-0011

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abstract = "A clear understanding of cell fate regulation during differentiation is key in successfully using stem cells for therapeutic applications. Here, we report that mild electrical stimulation strongly influences embryonic stem cells to assume a neuronal fate. Although the resulting neuronal cells showed no sign of specific terminal differentiation in culture, they showed potential to differentiate into various types of neurons in vivo, and, in adult mice, contributed to the injured spinal cord as neuronal cells. Induction of calcium ion influx is significant in this differentiation system. This phenomenon opens up possibilities for understanding novel mechanisms underlying cellular differentiation and early development, and, perhaps more importantly, suggests possibilities for treatments in medical contexts.",

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AU - Yamada, Masahisa

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AU - Iwanami, Akio

AU - Nakamura, Masaya

AU - Mizuno, Hideaki

AU - Ozawa, Michiru

AU - Ohyama-Goto, Ritsuko

AU - Kitamura, Naohito

AU - Kawano, Masako

AU - Tan-Takeuchi, Kyoko

AU - Ohtsuka, Chiho

AU - Miyawaki, Atsushi

AU - Takashima, Akihiko

AU - Ogawa, Masaharu

AU - Toyama, Yoshiaki

AU - Okano, Hideyuki

AU - Kondo, Takashi

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AB - A clear understanding of cell fate regulation during differentiation is key in successfully using stem cells for therapeutic applications. Here, we report that mild electrical stimulation strongly influences embryonic stem cells to assume a neuronal fate. Although the resulting neuronal cells showed no sign of specific terminal differentiation in culture, they showed potential to differentiate into various types of neurons in vivo, and, in adult mice, contributed to the injured spinal cord as neuronal cells. Induction of calcium ion influx is significant in this differentiation system. This phenomenon opens up possibilities for understanding novel mechanisms underlying cellular differentiation and early development, and, perhaps more importantly, suggests possibilities for treatments in medical contexts.

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KW - Embryonic stem cells

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KW - Tissue regeneration

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Electrical stimulation modulates fate determination of differentiating embryonic stem cells

Abstract

Keywords

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