Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system

Hitoshi Shiku; Shun Goto; Sungbong Jung; Kuniaki Nagamine; Masahiro Koide; Tomosato Itayama; Tomoyuki Yasukawa; Tomokazu Matsue

doi:10.1039/b808428a

Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system

Hitoshi Shiku, Shun Goto, Sungbong Jung, Kuniaki Nagamine, Masahiro Koide, Tomosato Itayama, Tomoyuki Yasukawa, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A highly sensitive and quantitative analysis was performed using a poly(dimethylsiloxane) (PDMS) microwell array in a scanning electrochemical microscopy setup. A microelectrode with a relatively large seal radius was used to cover the top of the cylindrical PDMS microwell (96 pL). The voltammogram for 4 mM ferrocyanide resulted in a charge value of 38 nC, suggesting that almost 100% of the reductant in the microwell was converted to the oxidation current. When genetically modified yeast cells were entrapped in the microwell, the accumulation of p-aminophenol (PAP) produced by expressing β-galactosidase (βGAL) was successfully observed.

Original language	English
Pages (from-to)	182-187
Number of pages	6
Journal	Analyst
Volume	134
Issue number	1
DOIs	https://doi.org/10.1039/b808428a
Publication status	Published - 2009 Jan 1

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Environmental Chemistry
Spectroscopy
Electrochemistry

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abstract = "A highly sensitive and quantitative analysis was performed using a poly(dimethylsiloxane) (PDMS) microwell array in a scanning electrochemical microscopy setup. A microelectrode with a relatively large seal radius was used to cover the top of the cylindrical PDMS microwell (96 pL). The voltammogram for 4 mM ferrocyanide resulted in a charge value of 38 nC, suggesting that almost 100% of the reductant in the microwell was converted to the oxidation current. When genetically modified yeast cells were entrapped in the microwell, the accumulation of p-aminophenol (PAP) produced by expressing β-galactosidase (βGAL) was successfully observed.",

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AU - Shiku, Hitoshi

AU - Goto, Shun

AU - Jung, Sungbong

AU - Nagamine, Kuniaki

AU - Koide, Masahiro

AU - Itayama, Tomosato

AU - Yasukawa, Tomoyuki

AU - Matsue, Tomokazu

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Electrochemical characterization of enzymatic activity of yeast cells entrapped in a poly(dimethylsiloxane) microwell on the basis of limited diffusion system

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