Electrochemical characterization of enzyme and immunoglobulin G patterned using microcontact printing

Hitoshi Shiku; Ayako Kumagai; Hong Qun Luo; Yasufumi Takahashi; Tomoyuki Yasukawa; Hiroshi Yamada; Tomokazu Matsue

doi:10.5796/electrochemistry.78.122

Electrochemical characterization of enzyme and immunoglobulin G patterned using microcontact printing

Hitoshi Shiku, Ayako Kumagai, Hong Qun Luo, Yasufumi Takahashi, Tomoyuki Yasukawa, Hiroshi Yamada, Tomokazu Matsue

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail We have electrochemically estimated enzyme concentration on glass slide patterned with μCP technique. We also estimated the enzyme concentration at the surface of poly(dimethylsiloxane) (PDMS), which corresponded to the enzyme activity at the PDMS stamp just before μCP. Our result suggested that it was possible to transfer enzyme monolayer from PDMS to glass surfaces with 100% efficiency with μCP. Immunoglobulin G (IgG) patterned with μCP was also characterized by tagging with enzyme labeled anti-IgG. Scanning electrochemical microscopy (SECM) image was obtained to visualize line and space pattern of IgG monolayer.

Original language	English
Pages (from-to)	122-125
Number of pages	4
Journal	Electrochemistry
Volume	78
Issue number	2
DOIs	https://doi.org/10.5796/electrochemistry.78.122
Publication status	Published - 2010 Feb

Keywords

Beta-galactosidase
Diaphorase
Microcontact printing
Scanning electrochemical microscopy

ASJC Scopus subject areas

Electrochemistry

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10.5796/electrochemistry.78.122

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title = "Electrochemical characterization of enzyme and immunoglobulin G patterned using microcontact printing",

abstract = "Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail We have electrochemically estimated enzyme concentration on glass slide patterned with μCP technique. We also estimated the enzyme concentration at the surface of poly(dimethylsiloxane) (PDMS), which corresponded to the enzyme activity at the PDMS stamp just before μCP. Our result suggested that it was possible to transfer enzyme monolayer from PDMS to glass surfaces with 100% efficiency with μCP. Immunoglobulin G (IgG) patterned with μCP was also characterized by tagging with enzyme labeled anti-IgG. Scanning electrochemical microscopy (SECM) image was obtained to visualize line and space pattern of IgG monolayer.",

keywords = "Beta-galactosidase, Diaphorase, Microcontact printing, Scanning electrochemical microscopy",

author = "Hitoshi Shiku and Ayako Kumagai and Luo, {Hong Qun} and Yasufumi Takahashi and Tomoyuki Yasukawa and Hiroshi Yamada and Tomokazu Matsue",

year = "2010",

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doi = "10.5796/electrochemistry.78.122",

language = "English",

volume = "78",

pages = "122--125",

journal = "Electrochemistry",

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publisher = "Electrochemical Society of Japan",

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TY - JOUR

T1 - Electrochemical characterization of enzyme and immunoglobulin G patterned using microcontact printing

AU - Shiku, Hitoshi

AU - Kumagai, Ayako

AU - Luo, Hong Qun

AU - Takahashi, Yasufumi

AU - Yasukawa, Tomoyuki

AU - Yamada, Hiroshi

AU - Matsue, Tomokazu

PY - 2010/2

Y1 - 2010/2

N2 - Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail We have electrochemically estimated enzyme concentration on glass slide patterned with μCP technique. We also estimated the enzyme concentration at the surface of poly(dimethylsiloxane) (PDMS), which corresponded to the enzyme activity at the PDMS stamp just before μCP. Our result suggested that it was possible to transfer enzyme monolayer from PDMS to glass surfaces with 100% efficiency with μCP. Immunoglobulin G (IgG) patterned with μCP was also characterized by tagging with enzyme labeled anti-IgG. Scanning electrochemical microscopy (SECM) image was obtained to visualize line and space pattern of IgG monolayer.

AB - Microcontact printing (μCP) with various proteins has been widely applied to biosensors and cell biology research. However, the mechanism of protein patterning in the μCP process is not clear in detail We have electrochemically estimated enzyme concentration on glass slide patterned with μCP technique. We also estimated the enzyme concentration at the surface of poly(dimethylsiloxane) (PDMS), which corresponded to the enzyme activity at the PDMS stamp just before μCP. Our result suggested that it was possible to transfer enzyme monolayer from PDMS to glass surfaces with 100% efficiency with μCP. Immunoglobulin G (IgG) patterned with μCP was also characterized by tagging with enzyme labeled anti-IgG. Scanning electrochemical microscopy (SECM) image was obtained to visualize line and space pattern of IgG monolayer.

KW - Beta-galactosidase

KW - Diaphorase

KW - Microcontact printing

KW - Scanning electrochemical microscopy

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Electrochemical characterization of enzyme and immunoglobulin G patterned using microcontact printing

Abstract

Keywords

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