@article{9ef8a56cfac3469a89e183caab96e7f2,
title = "Electrochemical imaging of cell activity in hydrogels embedded in grid-shaped polycaprolactone scaffolds using a large-scale integration-based amperometric device",
abstract = "Tissue engineering requires analytical methods to monitor cell activity in hydrogels. Here, we present a method for the electrochemical imaging of cell activity in hydrogels embedded in printed polycaprolactone (PCL) scaffolds. Because a structure made of only hydrogel is fragile, PCL frameworks are used as a support material. A grid-shaped PCL was fabricated using an excluder printer. Photocured hydrogels containing cells were set at each grid hole, and cell activity was monitored using a large-scale integration-based amperometric device. The electrochemical device contains 400 microelectrodes for biomolecule detection, such as dissolved oxygen and enzymatic products. As proof of the concept, alkaline phosphatase and respiration activities of embryonic stem cells in the hydrogels were electrochemically monitored. The results indicate that the electrochemical imaging is useful for evaluating cells in printed scaffolds.",
keywords = "Cell culture in hydrogels, Electrochemical bioimaging, Embryonic stem cell, LSI-based amperometric device, Multiple detection, Printed polycaprolactone",
author = "Kosuke Ino and Yuki Yokokawa and Noriko Taira and Atsushi Suda and Ryota Kunikata and Yuji Nashimoto and Tomokazu Matsue and Hitoshi Shiku",
note = "Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (A) (No. 16H02280), a Grant-in-Aid for Scientific Research (B) Nos. 15H03542, 18H01840, and 18H01999), a Grant-in-Aid for Challenging Exploratory Research (No. 16K14012), and a Grant-in-Aid for Young Scientists (A) (No. 15H05415) from the Japan Society for the Promotion of Science (JSPS). This work was also supported by the Asahi Glass Foundation and Program for Creation of Interdisciplinary Research from Frontier Research Institute for Interdisciplinary Sciences, Tohoku University. Funding Information: This work was supported by a Grant-in-Aid for Scientific Research (A) (No. 16H02280), a Grant-in-Aid for Scientific Research (B) Nos. 15H03542, 18H01840, and 18H01999), a Grant-in-Aid for Challenging Exploratory Research (No. 16K14012), and a Grant-in-Aid for Young Scientists (A) (No. 15H05415) from the Japan Society for the Promotion of Science (JSPS). This work was also supported by the Asahi Glass Foundation and Program for Creation of Interdisciplinary Research from Frontier Research Institute for Interdisciplinary Sciences, Tohoku University Publisher Copyright: {\textcopyright} The Japan Society for Analytical Chemistry.",
year = "2019",
doi = "10.2116/analsci.18SDP01",
language = "English",
volume = "35",
pages = "39--43",
journal = "Analytical Sciences",
issn = "0910-6340",
publisher = "Springer International Publishing AG",
number = "1",
}