TY - JOUR
T1 - Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors
AU - Abe, Hiroya
AU - Ino, Kosuke
AU - Li, Chen Zhong
AU - Kanno, Yusuke
AU - Inoue, Kumi Y.
AU - Suda, Atsushi
AU - Kunikata, Ryota
AU - Matsudaira, Masahki
AU - Takahashi, Yasufumi
AU - Shiku, Hitoshi
AU - Matsue, Tomokazu
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015/6/16
Y1 - 2015/6/16
N2 - In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 μm for rapid electrochemical imaging of large areas. PC12 spheroids were stimulated with K+ to release dopamine. Poststimulation dopamine release from the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bio-LSI clearly showed the effects of the dopaminergic drugs l-3,4-dihydroxyphenylalanine (l-DOPA) and reserpine on K+-stimulated dopamine release from PC12 spheroids. Our results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.
AB - In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 μm for rapid electrochemical imaging of large areas. PC12 spheroids were stimulated with K+ to release dopamine. Poststimulation dopamine release from the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bio-LSI clearly showed the effects of the dopaminergic drugs l-3,4-dihydroxyphenylalanine (l-DOPA) and reserpine on K+-stimulated dopamine release from PC12 spheroids. Our results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.
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U2 - 10.1021/acs.analchem.5b01307
DO - 10.1021/acs.analchem.5b01307
M3 - Article
C2 - 25971414
AN - SCOPUS:84934965149
SN - 0003-2700
VL - 87
SP - 6364
EP - 6370
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 12
ER -