Electrochemical nanoprobes for single-cell analysis

Paolo Actis, Sergiy Tokar, Jan Clausmeyer, Babak Babakinejad, Sofya Mikhaleva, Renaud Cornut, Yasufumi Takahashi, Ainara López Córdoba, Pavel Novak, Andrew I. Shevchuck, Jennifer A. Dougan, Sergei G. Kazarian, Petr V. Gorelkin, Alexander S. Erofeev, Igor V. Yaminsky, Patrick R. Unwin, Wolfgang Schuhmann, David Klenerman, Dmitri A. Rusakov, Elena V. SviderskayaYuri E. Korchev

    Research output: Contribution to journalArticlepeer-review

    169 Citations (Scopus)


    The measurement of key molecules in individual cells with minimal disruption to the biological milieu is the next frontier in single-cell analyses. Nanoscale devices are ideal analytical tools because of their small size and their potential for high spatial and temporal resolution recordings. Here, we report the fabrication of disk-shaped carbon nanoelectrodes whose radius can be precisely tuned within the range 5-200 nm. The functionalization of the nanoelectrode with platinum allowed the monitoring of oxygen consumption outside and inside a brain slice. Furthermore, we show that nanoelectrodes of this type can be used to impale individual cells to perform electrochemical measurements within the cell with minimal disruption to cell function. These nanoelectrodes can be fabricated combined with scanning ion conductance microscopy probes, which should allow high resolution electrochemical mapping of species on or in living cells.

    Original languageEnglish
    Pages (from-to)875-884
    Number of pages10
    JournalACS Nano
    Issue number1
    Publication statusPublished - 2014 Jan 28


    • brain slice
    • carbon
    • electrochemistry
    • intracellular measurements
    • melanoma
    • nanoelectrode
    • platinum

    ASJC Scopus subject areas

    • Materials Science(all)
    • Engineering(all)
    • Physics and Astronomy(all)


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