TY - JOUR
T1 - Electrochemicolor Imaging Using an LSI-Based Device for Multiplexed Cell Assays
AU - Kanno, Yusuke
AU - Ino, Kosuke
AU - Abe, Hiroya
AU - Sakamoto, Chika
AU - Onodera, Takehiro
AU - Inoue, Kumi Y.
AU - Suda, Atsushi
AU - Kunikata, Ryota
AU - Matsudaira, Masahki
AU - Shiku, Hitoshi
AU - Matsue, Tomokazu
N1 - Funding Information:
This work was supported in part by the Grant-in-Aid for Scientific Research (A) (No. 16H02280), Grant-in-Aid for Scientific Research (B) (No. 15H035420), Grant-in-Aid for Young Scientists (A) (No. 15H05415), and by the Grant-in-Aid for JSPS Fellows from the Japan Society for the Promotion of Science (JSPS). This work was also supported by the Special Coordination Funds for Promoting Science and Technology, Creation of Innovation Centers for Advanced Interdisciplinary Research Areas Program from the Japan Science and Technology Agency, the Asahi Glass Foundation, and by the Grant-in-Aid of the Tohoku University Institute for International Advanced Research and Education.
Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/12/5
Y1 - 2017/12/5
N2 - Multiplexed bioimaging systems have triggered the development of effective assays, contributing new biological information. Although electrochemical imaging is beneficial for quantitative analysis in real time, monitoring multiple cell functions is difficult. We have developed a novel electrochemical imaging system, herein, using a large-scale integration (LSI)-based amperometric device for detecting multiple biomolecules simultaneously. This system is designated as an electrochemicolor imaging system in which the current signals from two different types of biomolecules are depicted as a multicolor electrochemical image. The mode-selectable function of the 400-electrode device enables the imaging system and two different potentials can be independently applied to the selected electrodes. The imaging system is successfully applied for detecting multiple cell functions of the embryonic stem (ES) cell and the rat pheochromocytoma (PC12) cell aggregates. To the best of our knowledge, this is the first time that a real-time electrochemical mapping technique for multiple electroactive species, simultaneously, has been reported. The imaging system is a promising bioanalytical method for exploring complex biological phenomena.
AB - Multiplexed bioimaging systems have triggered the development of effective assays, contributing new biological information. Although electrochemical imaging is beneficial for quantitative analysis in real time, monitoring multiple cell functions is difficult. We have developed a novel electrochemical imaging system, herein, using a large-scale integration (LSI)-based amperometric device for detecting multiple biomolecules simultaneously. This system is designated as an electrochemicolor imaging system in which the current signals from two different types of biomolecules are depicted as a multicolor electrochemical image. The mode-selectable function of the 400-electrode device enables the imaging system and two different potentials can be independently applied to the selected electrodes. The imaging system is successfully applied for detecting multiple cell functions of the embryonic stem (ES) cell and the rat pheochromocytoma (PC12) cell aggregates. To the best of our knowledge, this is the first time that a real-time electrochemical mapping technique for multiple electroactive species, simultaneously, has been reported. The imaging system is a promising bioanalytical method for exploring complex biological phenomena.
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U2 - 10.1021/acs.analchem.7b03042
DO - 10.1021/acs.analchem.7b03042
M3 - Article
C2 - 29090905
AN - SCOPUS:85037536976
SN - 0003-2700
VL - 89
SP - 12778
EP - 12786
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 23
ER -