Electrophoretic manipulation and electrochemical analysis of single cell with the microfluidic device

Kuniaki Nagamine, Tomoyuki Yasukawa, Hitoshi Shiku, Masahiro Koide, Tomosato Itayama, Tomokazu Matsue

Research output: Contribution to conferencePaperpeer-review

Abstract

We have fabricated a microfluidic device comprising a 100 pL scale analytical chamber for electrochemical measurement of cellular activities. The microfluidic main channel and the analytical chamber were fabricated using poly(dimethylsiloxane) (PDMS). The chamber contained two Pt microelectrodes served as an electrochemical working electrode and an electrophoretic electrode to manipulate cells into the chamber. Yeast cells were introduced to the chamber by the electrophoresis, and its β-galactosidase (βGal) activities were characterized by amperometry. p-Aminophenol (PAP), generated by the enzyme-catalyzed hydrolysis of p-aminophenyl-β-D-galactopyranoside (PAPG), was detected electrochemically.

Original languageEnglish
Pages645-647
Number of pages3
Publication statusPublished - 2006
Event10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
Duration: 2006 Nov 52006 Nov 9

Conference

Conference10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
Country/TerritoryJapan
CityTokyo
Period06/11/506/11/9

Keywords

  • Electrochemistry
  • Electrophoresis
  • Microfluidic device
  • Single cell

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