Electrophysiological properties of NtTPK1 expressed in yeast tonoplast

Shin Hamamoto; Isamu Yabe; Nobuyuki Uozumi

doi:10.1271/bbb.80354

Electrophysiological properties of NtTPK1 expressed in yeast tonoplast

Shin Hamamoto, Isamu Yabe, Nobuyuki Uozumi

ENG - Biomolecular Engineering

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The patch clamp technique using enlarged yeast tonoplasts is an effective approach to characterizing the Nicotiana tabacum plant tonoplast K⁺ channel, NtTPK1. We report here that the NtTPK1-mediated currents comprise two phase components, both which were found to be highly selective for K⁺ over Na⁺ and Cl^-.

Original language	English
Pages (from-to)	2785-2787
Number of pages	3
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	10
DOIs	https://doi.org/10.1271/bbb.80354
Publication status	Published - 2008

Keywords

Channel
Patch clamp
Potassium
Tobacco
Yeast

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10.1271/bbb.80354

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title = "Electrophysiological properties of NtTPK1 expressed in yeast tonoplast",

abstract = "The patch clamp technique using enlarged yeast tonoplasts is an effective approach to characterizing the Nicotiana tabacum plant tonoplast K+ channel, NtTPK1. We report here that the NtTPK1-mediated currents comprise two phase components, both which were found to be highly selective for K+ over Na+ and Cl-.",

keywords = "Channel, Patch clamp, Potassium, Tobacco, Yeast",

author = "Shin Hamamoto and Isamu Yabe and Nobuyuki Uozumi",

note = "Funding Information: This work was supported by Grants-in-Aid for Scientific Research (nos. 19380058 and 17078005) to N.U. from Ministry of Education, Culture, Sports, Science and Technology and Japan Society for the Promotion of Science.",

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doi = "10.1271/bbb.80354",

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publisher = "Oxford University Press",

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T1 - Electrophysiological properties of NtTPK1 expressed in yeast tonoplast

AU - Hamamoto, Shin

AU - Yabe, Isamu

AU - Uozumi, Nobuyuki

N1 - Funding Information: This work was supported by Grants-in-Aid for Scientific Research (nos. 19380058 and 17078005) to N.U. from Ministry of Education, Culture, Sports, Science and Technology and Japan Society for the Promotion of Science.

PY - 2008

Y1 - 2008

N2 - The patch clamp technique using enlarged yeast tonoplasts is an effective approach to characterizing the Nicotiana tabacum plant tonoplast K+ channel, NtTPK1. We report here that the NtTPK1-mediated currents comprise two phase components, both which were found to be highly selective for K+ over Na+ and Cl-.

AB - The patch clamp technique using enlarged yeast tonoplasts is an effective approach to characterizing the Nicotiana tabacum plant tonoplast K+ channel, NtTPK1. We report here that the NtTPK1-mediated currents comprise two phase components, both which were found to be highly selective for K+ over Na+ and Cl-.

KW - Channel

KW - Patch clamp

KW - Potassium

KW - Tobacco

KW - Yeast

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UR - http://www.scopus.com/inward/citedby.url?scp=55049096725&partnerID=8YFLogxK

U2 - 10.1271/bbb.80354

DO - 10.1271/bbb.80354

M3 - Article

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AN - SCOPUS:55049096725

SN - 0916-8451

VL - 72

SP - 2785

EP - 2787

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 10

ER -

Electrophysiological properties of NtTPK1 expressed in yeast tonoplast

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Keywords

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