Electroporation in the rodent embryonic brain using whole embryo culture system

Takako Kikkawa; Masanori Takahashi; Noriko Osumi

doi:10.1002/cpns.21

Electroporation in the rodent embryonic brain using whole embryo culture system

Takako Kikkawa, Masanori Takahashi, Noriko Osumi

MED - United Centers for Advanced Research and Translational Medicine (ART)

Jichi Medical University

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This unit describes basic methods formammalianwhole embryo culture (WEC) using embryonic day 10.5 mouse embryos, including the preparation of highquality immediately centrifuged (IC) rat serum that is commonly used for WEC and is essential for normal growth and development of cultured mouse and rat embryos in vitro. An alternative protocol for different stages of rodent embryos is also introduced. Since embryos for WEC are dissected out of the uterus and manipulated under the microscope, one can overcome many of the difficulties of gene delivery encountered using in utero electroporation. A description for a gene transfer method to label neural stem/progenitor cells of the cortical primordium in a highly region-specific manner is also included.

Original language	English
Pages (from-to)	3.30.1-3.30.16
Journal	Current Protocols in Neuroscience
Volume	2017
DOIs	https://doi.org/10.1002/cpns.21
Publication status	Published - 2017 Jan 1

Keywords

Brain development
Electroporation
Whole embryo culture

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10.1002/cpns.21

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title = "Electroporation in the rodent embryonic brain using whole embryo culture system",

abstract = "This unit describes basic methods formammalianwhole embryo culture (WEC) using embryonic day 10.5 mouse embryos, including the preparation of highquality immediately centrifuged (IC) rat serum that is commonly used for WEC and is essential for normal growth and development of cultured mouse and rat embryos in vitro. An alternative protocol for different stages of rodent embryos is also introduced. Since embryos for WEC are dissected out of the uterus and manipulated under the microscope, one can overcome many of the difficulties of gene delivery encountered using in utero electroporation. A description for a gene transfer method to label neural stem/progenitor cells of the cortical primordium in a highly region-specific manner is also included.",

keywords = "Brain development, Electroporation, Whole embryo culture",

author = "Takako Kikkawa and Masanori Takahashi and Noriko Osumi",

note = "Funding Information: We would like to thank Dr. Tetsuichiro Saito for providing pCAG-EGFP plasmid. We are grateful to Ms. Sayaka Makino for animal care and technical support. This work was supported by KAKENHI (#26291046 and #16H06530 to N.O. and #16K20902 to T.K.). Publisher Copyright: {\textcopyright} 2017 by John Wiley & Sons, Inc.",

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doi = "10.1002/cpns.21",

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AU - Takahashi, Masanori

AU - Osumi, Noriko

N1 - Funding Information: We would like to thank Dr. Tetsuichiro Saito for providing pCAG-EGFP plasmid. We are grateful to Ms. Sayaka Makino for animal care and technical support. This work was supported by KAKENHI (#26291046 and #16H06530 to N.O. and #16K20902 to T.K.). Publisher Copyright: © 2017 by John Wiley & Sons, Inc.

PY - 2017/1/1

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N2 - This unit describes basic methods formammalianwhole embryo culture (WEC) using embryonic day 10.5 mouse embryos, including the preparation of highquality immediately centrifuged (IC) rat serum that is commonly used for WEC and is essential for normal growth and development of cultured mouse and rat embryos in vitro. An alternative protocol for different stages of rodent embryos is also introduced. Since embryos for WEC are dissected out of the uterus and manipulated under the microscope, one can overcome many of the difficulties of gene delivery encountered using in utero electroporation. A description for a gene transfer method to label neural stem/progenitor cells of the cortical primordium in a highly region-specific manner is also included.

AB - This unit describes basic methods formammalianwhole embryo culture (WEC) using embryonic day 10.5 mouse embryos, including the preparation of highquality immediately centrifuged (IC) rat serum that is commonly used for WEC and is essential for normal growth and development of cultured mouse and rat embryos in vitro. An alternative protocol for different stages of rodent embryos is also introduced. Since embryos for WEC are dissected out of the uterus and manipulated under the microscope, one can overcome many of the difficulties of gene delivery encountered using in utero electroporation. A description for a gene transfer method to label neural stem/progenitor cells of the cortical primordium in a highly region-specific manner is also included.

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JF - Current Protocols in Neuroscience

ER -

Electroporation in the rodent embryonic brain using whole embryo culture system

Abstract

Keywords

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