Electroporation of adherent cells by direct lamination of hydrogel-based microelectrode substrates

Daisuke Takahashi; Masato Sasaki; Kuniaki Nagamine; Keiichi Torimitsu; Matsuhiko Nishizawa

doi:10.1246/cl.131083

Electroporation of adherent cells by direct lamination of hydrogel-based microelectrode substrates

Daisuke Takahashi, Masato Sasaki, Kuniaki Nagamine, Keiichi Torimitsu, Matsuhiko Nishizawa

Tohoku University

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

We have developed a technique to fabricate Au microelectrodes on hydrogel substrates such as polyacrylamide. Electropolymerization of poly(3,4- ethylenedioxythiophene) (PEDOT) anchors the Au electrodes to the hydrogels. The resulting soft, molecularly permeable hydrogel-based microelectrodes were effective for the electroporation of adherent cells by direct lamination. The electroporation-assisted uptakes of dye molecules and genes were demonstrated for normal human dermal fibroblast cells and human induced pluripotent stem (iPS) cells.

Original language	English
Pages (from-to)	444-446
Number of pages	3
Journal	Chemistry Letters
Volume	43
Issue number	4
DOIs	https://doi.org/10.1246/cl.131083
Publication status	Published - 2014

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abstract = "We have developed a technique to fabricate Au microelectrodes on hydrogel substrates such as polyacrylamide. Electropolymerization of poly(3,4- ethylenedioxythiophene) (PEDOT) anchors the Au electrodes to the hydrogels. The resulting soft, molecularly permeable hydrogel-based microelectrodes were effective for the electroporation of adherent cells by direct lamination. The electroporation-assisted uptakes of dye molecules and genes were demonstrated for normal human dermal fibroblast cells and human induced pluripotent stem (iPS) cells.",

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AU - Takahashi, Daisuke

AU - Sasaki, Masato

AU - Nagamine, Kuniaki

AU - Torimitsu, Keiichi

AU - Nishizawa, Matsuhiko

PY - 2014

Y1 - 2014

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AB - We have developed a technique to fabricate Au microelectrodes on hydrogel substrates such as polyacrylamide. Electropolymerization of poly(3,4- ethylenedioxythiophene) (PEDOT) anchors the Au electrodes to the hydrogels. The resulting soft, molecularly permeable hydrogel-based microelectrodes were effective for the electroporation of adherent cells by direct lamination. The electroporation-assisted uptakes of dye molecules and genes were demonstrated for normal human dermal fibroblast cells and human induced pluripotent stem (iPS) cells.

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JO - Chemistry Letters

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Electroporation of adherent cells by direct lamination of hydrogel-based microelectrode substrates

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