Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer

Shunji Ishiwata; Yasuko Ozawa; Jun Katayama; Shuko Kaneko; Hiroki Shindo; Yoshihisa Tomioka; Toshiyuki Ishiwata; Goro Asano; Shigeo Ikegawa; Michinao Mizugaki

doi:10.1016/j.canlet.2004.03.009

Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer

Shunji Ishiwata, Yasuko Ozawa, Jun Katayama, Shuko Kaneko, Hiroki Shindo, Yoshihisa Tomioka, Toshiyuki Ishiwata, Goro Asano, Shigeo Ikegawa, Michinao Mizugaki

PHA - Pharmacy

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

tRNA-guanine transglycosylase (TGT) is an enzyme which synthesizes a modified nucleoside, queuosine, by exchanging the base moiety of guanosine for queuine in tRNA. We have reported that the expression level of the 60-kDa subunit of TGT (TGT60kD) is elevated in leukemic cells, however, there is no other report on the expression of TGT60kD in cancer cells. The expression levels of the TGT60kD protein are elevated in four of the five colon cancer cell lines and 83% of colon cancer tissues compared with normal tissues. The expression levels of the TGT60kD protein decreased in two colon cancer cell lines, after cell differentiation was induced. A marked positive staining of cancer cells in colon tissues was observed, and the subcellular staining pattern was mainly cytosolic. These data suggest that the role of TGT60kD in colon carcinogenesis.

Original language	English
Pages (from-to)	113-119
Number of pages	7
Journal	Cancer Letters
Volume	212
Issue number	1
DOIs	https://doi.org/10.1016/j.canlet.2004.03.009
Publication status	Published - 2004 Aug 20

Keywords

Colon cancer
Differentiation
Expression
Queuosine
tRNA-guanine transglycosylase

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.canlet.2004.03.009

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title = "Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer",

abstract = "tRNA-guanine transglycosylase (TGT) is an enzyme which synthesizes a modified nucleoside, queuosine, by exchanging the base moiety of guanosine for queuine in tRNA. We have reported that the expression level of the 60-kDa subunit of TGT (TGT60kD) is elevated in leukemic cells, however, there is no other report on the expression of TGT60kD in cancer cells. The expression levels of the TGT60kD protein are elevated in four of the five colon cancer cell lines and 83% of colon cancer tissues compared with normal tissues. The expression levels of the TGT60kD protein decreased in two colon cancer cell lines, after cell differentiation was induced. A marked positive staining of cancer cells in colon tissues was observed, and the subcellular staining pattern was mainly cytosolic. These data suggest that the role of TGT60kD in colon carcinogenesis.",

keywords = "Colon cancer, Differentiation, Expression, Queuosine, tRNA-guanine transglycosylase",

author = "Shunji Ishiwata and Yasuko Ozawa and Jun Katayama and Shuko Kaneko and Hiroki Shindo and Yoshihisa Tomioka and Toshiyuki Ishiwata and Goro Asano and Shigeo Ikegawa and Michinao Mizugaki",

year = "2004",

month = aug,

day = "20",

doi = "10.1016/j.canlet.2004.03.009",

language = "English",

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journal = "Cancer Letters",

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T1 - Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer

AU - Ishiwata, Shunji

AU - Ozawa, Yasuko

AU - Katayama, Jun

AU - Kaneko, Shuko

AU - Shindo, Hiroki

AU - Tomioka, Yoshihisa

AU - Ishiwata, Toshiyuki

AU - Asano, Goro

AU - Ikegawa, Shigeo

AU - Mizugaki, Michinao

PY - 2004/8/20

Y1 - 2004/8/20

N2 - tRNA-guanine transglycosylase (TGT) is an enzyme which synthesizes a modified nucleoside, queuosine, by exchanging the base moiety of guanosine for queuine in tRNA. We have reported that the expression level of the 60-kDa subunit of TGT (TGT60kD) is elevated in leukemic cells, however, there is no other report on the expression of TGT60kD in cancer cells. The expression levels of the TGT60kD protein are elevated in four of the five colon cancer cell lines and 83% of colon cancer tissues compared with normal tissues. The expression levels of the TGT60kD protein decreased in two colon cancer cell lines, after cell differentiation was induced. A marked positive staining of cancer cells in colon tissues was observed, and the subcellular staining pattern was mainly cytosolic. These data suggest that the role of TGT60kD in colon carcinogenesis.

AB - tRNA-guanine transglycosylase (TGT) is an enzyme which synthesizes a modified nucleoside, queuosine, by exchanging the base moiety of guanosine for queuine in tRNA. We have reported that the expression level of the 60-kDa subunit of TGT (TGT60kD) is elevated in leukemic cells, however, there is no other report on the expression of TGT60kD in cancer cells. The expression levels of the TGT60kD protein are elevated in four of the five colon cancer cell lines and 83% of colon cancer tissues compared with normal tissues. The expression levels of the TGT60kD protein decreased in two colon cancer cell lines, after cell differentiation was induced. A marked positive staining of cancer cells in colon tissues was observed, and the subcellular staining pattern was mainly cytosolic. These data suggest that the role of TGT60kD in colon carcinogenesis.

KW - Colon cancer

KW - Differentiation

KW - Expression

KW - Queuosine

KW - tRNA-guanine transglycosylase

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DO - 10.1016/j.canlet.2004.03.009

M3 - Article

C2 - 15246567

AN - SCOPUS:3042815958

SN - 0304-3835

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SP - 113

EP - 119

JO - Cancer Letters

JF - Cancer Letters

IS - 1

ER -

Elevated expression level of 60-kDa subunit of tRNA-guanine transglycosylase in colon cancer

Abstract

Keywords

UN SDGs

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