Elucidation of the TMab-6 Monoclonal Antibody Epitope Against Telomerase Reverse Transcriptase

Mika K. Kaneko; Shinji Yamada; Shunsuke Itai; Yao Wen Chang; Takuro Nakamura; Miyuki Yanaka; Hiroyuki Harada; Hiroyoshi Suzuki; Yukinari Kato

doi:10.1089/mab.2018.0007

Elucidation of the TMab-6 Monoclonal Antibody Epitope Against Telomerase Reverse Transcriptase

Mika K. Kaneko, Shinji Yamada, Shunsuke Itai, Yao Wen Chang, Takuro Nakamura, Miyuki Yanaka, Hiroyuki Harada, Hiroyoshi Suzuki, Yukinari Kato

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Telomerase reverse transcriptase (TERT) and mutations of the TERT promoter are significant in the pathogenesis of 1p/19q-codeleted oligodendrogliomas and isocitrate dehydrogenase gene wild-type glioblastomas, as well as melanomas and squamous cell carcinomas. We previously developed an antihuman TERT monoclonal antibody (mAb), TMab-6, which is applicable in immunohistochemistry for human tissues. However, the binding epitope of TMab-6 against TERT is yet to be elucidated. In this study, enzyme-linked immunosorbent assay and immunohistochemistry were utilized for investigating the epitope of TMab-6. The findings revealed that the critical epitope of TMab-6 is the TERT sequence PSTSRPPRPWD; Thr310 and Ser311 of TERT are especially significant amino acids for TMab-6 recognition.

Original language	English
Pages (from-to)	101-103
Number of pages	3
Journal	Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
Volume	38
Issue number	3
DOIs	https://doi.org/10.1089/mab.2018.0007
Publication status	Published - 2019 Jun

Keywords

epitope mapping
monoclonal antibody
TERT

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10.1089/mab.2018.0007

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title = "Elucidation of the TMab-6 Monoclonal Antibody Epitope Against Telomerase Reverse Transcriptase",

abstract = "Telomerase reverse transcriptase (TERT) and mutations of the TERT promoter are significant in the pathogenesis of 1p/19q-codeleted oligodendrogliomas and isocitrate dehydrogenase gene wild-type glioblastomas, as well as melanomas and squamous cell carcinomas. We previously developed an antihuman TERT monoclonal antibody (mAb), TMab-6, which is applicable in immunohistochemistry for human tissues. However, the binding epitope of TMab-6 against TERT is yet to be elucidated. In this study, enzyme-linked immunosorbent assay and immunohistochemistry were utilized for investigating the epitope of TMab-6. The findings revealed that the critical epitope of TMab-6 is the TERT sequence PSTSRPPRPWD; Thr310 and Ser311 of TERT are especially significant amino acids for TMab-6 recognition.",

keywords = "epitope mapping, monoclonal antibody, TERT",

author = "Kaneko, {Mika K.} and Shinji Yamada and Shunsuke Itai and Chang, {Yao Wen} and Takuro Nakamura and Miyuki Yanaka and Hiroyuki Harada and Hiroyoshi Suzuki and Yukinari Kato",

note = "Funding Information: We thank Noriko Saidoh, Saori Handa, and Yoshimi Nakamura for their excellent technical assistance. This research was supported, in part, by AMED under Grant Numbers: JP17am0301010 (Y.K.), JP17am0101078 (Y.K.), and JP17ae0101028 (Y.K.), and by JSPS KAKENHI Grant Number 17K07299 (M.K.K.) and Grant Number 16K10748 (Y.K.). Publisher Copyright: {\textcopyright} 2019, Mary Ann Liebert, Inc., publishers 2019.",

year = "2019",

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doi = "10.1089/mab.2018.0007",

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AU - Kaneko, Mika K.

AU - Yamada, Shinji

AU - Itai, Shunsuke

AU - Chang, Yao Wen

AU - Nakamura, Takuro

AU - Yanaka, Miyuki

AU - Harada, Hiroyuki

AU - Suzuki, Hiroyoshi

AU - Kato, Yukinari

N1 - Funding Information: We thank Noriko Saidoh, Saori Handa, and Yoshimi Nakamura for their excellent technical assistance. This research was supported, in part, by AMED under Grant Numbers: JP17am0301010 (Y.K.), JP17am0101078 (Y.K.), and JP17ae0101028 (Y.K.), and by JSPS KAKENHI Grant Number 17K07299 (M.K.K.) and Grant Number 16K10748 (Y.K.). Publisher Copyright: © 2019, Mary Ann Liebert, Inc., publishers 2019.

PY - 2019/6

Y1 - 2019/6

N2 - Telomerase reverse transcriptase (TERT) and mutations of the TERT promoter are significant in the pathogenesis of 1p/19q-codeleted oligodendrogliomas and isocitrate dehydrogenase gene wild-type glioblastomas, as well as melanomas and squamous cell carcinomas. We previously developed an antihuman TERT monoclonal antibody (mAb), TMab-6, which is applicable in immunohistochemistry for human tissues. However, the binding epitope of TMab-6 against TERT is yet to be elucidated. In this study, enzyme-linked immunosorbent assay and immunohistochemistry were utilized for investigating the epitope of TMab-6. The findings revealed that the critical epitope of TMab-6 is the TERT sequence PSTSRPPRPWD; Thr310 and Ser311 of TERT are especially significant amino acids for TMab-6 recognition.

AB - Telomerase reverse transcriptase (TERT) and mutations of the TERT promoter are significant in the pathogenesis of 1p/19q-codeleted oligodendrogliomas and isocitrate dehydrogenase gene wild-type glioblastomas, as well as melanomas and squamous cell carcinomas. We previously developed an antihuman TERT monoclonal antibody (mAb), TMab-6, which is applicable in immunohistochemistry for human tissues. However, the binding epitope of TMab-6 against TERT is yet to be elucidated. In this study, enzyme-linked immunosorbent assay and immunohistochemistry were utilized for investigating the epitope of TMab-6. The findings revealed that the critical epitope of TMab-6 is the TERT sequence PSTSRPPRPWD; Thr310 and Ser311 of TERT are especially significant amino acids for TMab-6 recognition.

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Elucidation of the TMab-6 Monoclonal Antibody Epitope Against Telomerase Reverse Transcriptase

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Keywords

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