In the loop: Engineering of the surface loop in haloalkane dehalogenases affects their enantiodiscrimination behavior. The temperature dependence of the enantioselectivity (lnE versus 1/T) of β-bromoalkanes by haloalkane dehalogenases is reversed (red data points) by deletion of the surface loop; the selectivity switches back when an additional single-point mutation is made. This behavior is not observed for -bromoesters.
|Number of pages||5|
|Journal||Angewandte Chemie - International Edition|
|Publication status||Published - 2010 Aug 16|
- Haloalkane dehalogenases
- Protein engineering
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