Endothelin immunoreactivity in mice with Gram-negative bacteraemia: Relationship to tumour necrosis factor-α

K. Takahashi, A. Silva, J. Cohen, H. C. Lam, M. A. Ghatei, S. R. Bloom

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19 Citations (Scopus)


To investigate the role of endothelin in Gram-negative bacteraemia and the possible involvement of tumour necrosis factor-α in its pathophysiology, we measured plasma and tissue (lung, kidney and spleen) immunoreactive endothelin levels in Gram-negative bacteraemic mice, with and without passive immunization by anti-(tumour necrosis factor-α) antibody. Plasma immunoreactive endothelin levels were greatly increased after the Escherichia coli injection. Pretreatment with anti-(tumour necrosis factor-α) antibody did not suppress elevated plasma immunoreactive endothelin levels (P > 0.1). Lung tissue immunoreactive endothelin levels in mice were increased 16 h after the E. coli injection and were not affected by prior passive immunization with anti(-tumour necrosis factor-α) antibody. Immunoreactive endothelin in spleen and kidney was undetectable (< 34 fmol/g wet weight). Injection of rMu tumour necrosis factor-α into mice did not increase plasma immunoreactive endothelin levels. Antibody to endothelin given 30 min after a 90% lethal dose challenge with E. coli did not affect mortality. We conclude that the rise in plasma and tissue endothelin that occurs in Gram-negative bacteraemia is independent of tumor necrosis factor-α.

Original languageEnglish
Pages (from-to)619-623
Number of pages5
JournalClinical Science
Issue number6
Publication statusPublished - 1990


  • bacteraemia
  • chromatography
  • endothelin
  • endotoxin
  • Escherichia coli
  • gram-negative bacteraemia
  • lipopolysaccharide
  • tumour necrosis factor-α


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