Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1, for immunotherapy

Takashi Okada, Ines Swoboda, Prem L. Bhalla, Kinya Toriyama, Mohan B. Singh

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)


The Brassica pollen allergen Bra r 1 belongs to a new family of Ca2+-binding proteins, characterized by the presence of two potential EF-hand calcium-binding domains. Disruption of these EF-hand motifs by amino acid substitutions demonstrated that both domains of Bra r 1 constitute functional Ca2+-binding sites. Calcium-binding deficient mutants displayed significantly reduced IgE-binding activity. Injection of these mutated Bra r 1 variants into a murine model system showed that mouse IgG raised against the mutants recognized native Bra r 1 in Brassica pollen extracts suggesting the potential use of the engineered allergens for effective immunotherapy. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)255-260
Number of pages6
JournalFEBS Letters
Issue number3
Publication statusPublished - 1998 Sept 4


  • Brassica rapa
  • Ca binding
  • IgE reactivity
  • Mutagenesis analysis
  • Pollen allergen
  • Recombinant protein


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