Abstract
Objective: To elucidate the effect of the third variable (V3) loop in the early entry stage of human immunodeficiency virus type 1 (HIV-1). Methods: Three looped V3 peptides (V3-BH10, V3-ADA, V3-89.6) derived from three different HIV strains BH10 (X4), ADA (R5), 89.6(R5X4) were made. A non-loop control peptide V3-BH10/CA and its shorter forms V3-NNT-24 as well as V3-IRI12 were also synthesized. Three lentivirus-based luciferase reporter pseudoviruses bearing the envelop of HIV-1 HXB2, ADA or 89.6, respectively, were constructed. The effects of the peptides on the entry of various-tropic HIV-1 strains into target cells were examined. Results: (1) V3 peptides enhanced the entry of HIV-1 pseudoviruses HXB2, ADA, and 89.6 in a manner of virus-restriction. (2) Similar enhancement effect of linear peptide, V3-BH10/CA, was also found; its shorter form, V3-NNT24, which contains C-terminal of V3-BH10/CA, showed similar effect, while V2-IRI12, its shorter form containing only the central part did not show enhancement. Conclusion: The present study finds that HIV-1 V3 loop can enhance viral entry into target cells in a manner of strain-specificity for the first time. This finding will facilitate to understand the mechanism of HIV entry.
| Original language | English |
|---|---|
| Pages (from-to) | 275-278 |
| Number of pages | 4 |
| Journal | Chinese Journal of Microbiology and Immunology |
| Volume | 24 |
| Issue number | 4 |
| Publication status | Published - 2004 Apr 1 |
Keywords
- Human immunodeficiency virus type 1 (HIV-1)
- Peptide
- Target cells
- V3 loop
- Virus entry
ASJC Scopus subject areas
- Microbiology
- Immunology
- Virology
