TY - JOUR
T1 - Enhanced uptake of giant DNA in cell-sized liposomes
AU - Sato, Yuko
AU - Nomura, Shin Ichiro M.
AU - Yoshikawa, Kenichi
PY - 2003/10/21
Y1 - 2003/10/21
N2 - We have studied the spontaneous formation of giant liposome from dry lipid film under an aqueous solution of long DNA molecules, T4DNA 165 kbp. The number density of DNAs entrapped inside liposomes formed with 10 mM Mg2+ solution is one order of magnitude higher than that in the bulk solution. In addition, DNA molecules assume a somewhat shrunken conformation in phospholipid solution containing Mg2+. The mechanism of the enhanced uptake of DNA into cell-sized liposomes is discussed in relation to the local increase in osmotic pressure due to DNA molecules attached to the membrane surface through Mg2+.
AB - We have studied the spontaneous formation of giant liposome from dry lipid film under an aqueous solution of long DNA molecules, T4DNA 165 kbp. The number density of DNAs entrapped inside liposomes formed with 10 mM Mg2+ solution is one order of magnitude higher than that in the bulk solution. In addition, DNA molecules assume a somewhat shrunken conformation in phospholipid solution containing Mg2+. The mechanism of the enhanced uptake of DNA into cell-sized liposomes is discussed in relation to the local increase in osmotic pressure due to DNA molecules attached to the membrane surface through Mg2+.
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U2 - 10.1016/j.cplett.2003.08.050
DO - 10.1016/j.cplett.2003.08.050
M3 - Article
AN - SCOPUS:0142042949
SN - 0009-2614
VL - 380
SP - 279
EP - 285
JO - Chemical Physics Letters
JF - Chemical Physics Letters
IS - 3-4
ER -