Enhancement of mRNA Synthesis from Marek's Disease Virus Genome in the Lymphoblas-toid Cell Line, MDCC-MSB1, by 5-Azacytidine

Masanobu Hayashi; Tatsuya Furuichi; Shaomin Ren; Shigeo Namioka; Emiko Isogai; Meihan Nonoyama

doi:10.1292/jvms.56.305

Enhancement of mRNA Synthesis from Marek's Disease Virus Genome in the Lymphoblas-toid Cell Line, MDCC-MSB1, by 5-Azacytidine

Masanobu Hayashi, Tatsuya Furuichi, Shaomin Ren, Shigeo Namioka, Emiko Isogai, Meihan Nonoyama

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. A treatment of the MDV-derived lymphoblastoid cell line, MDCC-MSB1 (MSB1), with 5-azacytidine (5-AzC) resulted in a hypomethylation of MDV DNA and an increase in mRNA from certain portions of the MDV DNA. These results suggest methylation of MDV DNA as being one of the factors associated with a repression of transcription of MDV DNA in the lymphoblastoid cell line, MSB1.

Original language	English
Pages (from-to)	305-308
Number of pages	4
Journal	Journal of Veterinary Medical Science
Volume	56
Issue number	2
DOIs	https://doi.org/10.1292/jvms.56.305
Publication status	Published - 1994
Externally published	Yes

Keywords

5-AzC
MDV DNA
lymphoblastoid cell
mRNA synthesis
methylation

ASJC Scopus subject areas

veterinary(all)

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10.1292/jvms.56.305

Cite this

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title = "Enhancement of mRNA Synthesis from Marek's Disease Virus Genome in the Lymphoblas-toid Cell Line, MDCC-MSB1, by 5-Azacytidine",

abstract = "Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. A treatment of the MDV-derived lymphoblastoid cell line, MDCC-MSB1 (MSB1), with 5-azacytidine (5-AzC) resulted in a hypomethylation of MDV DNA and an increase in mRNA from certain portions of the MDV DNA. These results suggest methylation of MDV DNA as being one of the factors associated with a repression of transcription of MDV DNA in the lymphoblastoid cell line, MSB1.",

keywords = "5-AzC, MDV DNA, lymphoblastoid cell, mRNA synthesis, methylation",

author = "Masanobu Hayashi and Tatsuya Furuichi and Shaomin Ren and Shigeo Namioka and Emiko Isogai and Meihan Nonoyama",

year = "1994",

doi = "10.1292/jvms.56.305",

language = "English",

volume = "56",

pages = "305--308",

journal = "Journal of Veterinary Medical Science",

issn = "0916-7250",

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T1 - Enhancement of mRNA Synthesis from Marek's Disease Virus Genome in the Lymphoblas-toid Cell Line, MDCC-MSB1, by 5-Azacytidine

AU - Hayashi, Masanobu

AU - Furuichi, Tatsuya

AU - Ren, Shaomin

AU - Namioka, Shigeo

AU - Isogai, Emiko

AU - Nonoyama, Meihan

PY - 1994

Y1 - 1994

N2 - Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. A treatment of the MDV-derived lymphoblastoid cell line, MDCC-MSB1 (MSB1), with 5-azacytidine (5-AzC) resulted in a hypomethylation of MDV DNA and an increase in mRNA from certain portions of the MDV DNA. These results suggest methylation of MDV DNA as being one of the factors associated with a repression of transcription of MDV DNA in the lymphoblastoid cell line, MSB1.

AB - Marek's disease virus (MDV) DNA in latently infected lymphoblastoid cell lines is considerably methylated. A treatment of the MDV-derived lymphoblastoid cell line, MDCC-MSB1 (MSB1), with 5-azacytidine (5-AzC) resulted in a hypomethylation of MDV DNA and an increase in mRNA from certain portions of the MDV DNA. These results suggest methylation of MDV DNA as being one of the factors associated with a repression of transcription of MDV DNA in the lymphoblastoid cell line, MSB1.

KW - 5-AzC

KW - MDV DNA

KW - lymphoblastoid cell

KW - mRNA synthesis

KW - methylation

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Enhancement of mRNA Synthesis from Marek's Disease Virus Genome in the Lymphoblas-toid Cell Line, MDCC-MSB1, by 5-Azacytidine

Abstract

Keywords

ASJC Scopus subject areas

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